Bioremediation of dioxins with dioxins degradable fungi and its fusants formed by protoplast fusion of the fungi

二恶英降解真菌及其原生质体融合体对二恶英的生物修复

基本信息

批准号：
11660166
负责人：
TACHIBANA Sanro
金额：
$ 2.11万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660166/
关键词：
Dioxins Screening Bioremediation Protoplast fusion Incinerated ashes Contaminated soils Purification of Environment Dioxins degradable fungi 土壌

项目摘要

Biodegradation of dioxins in soils and incinerated ashes by bioremediation was conducted by use of dioxins degradable fungi and its fusants formed by protoplast fusion of the fungi. And, formation of fusants having higher ability for degradation of dioxins formed by the fusion of the fungi were also conducted.1. Each dioxin (2, 7-DCDD, 2, 4, 8-TCDF and 2, 3, 7, 8-TCDD) in soils could be degraded by bioremediation with dioxins degradable fungi. Each dioxin was degraded about 70%, 65% and 60%, respectively, for 30 days.2. About 90% of dioxins in paddy filed soils was degraded by bioremediation with dioxins degradable fungi for 30 days. The toxicity of the dioxins in the solis was alos removed by the bioremediation. And furthermore, the degree of degradation of the dioxins was increased by addition of a surfactant during the bioremediation.3. About 50% of dioxins in nicinerated ashes was degraded by bioremediation with dioxins degradable fungi for 60 days. It was found that the degree of the dioxins in the ashes was considerably increased by repetition of the bioremediation.4. Dioxins in paddy filed soils could be degraded by bioremediation with fusants formed by prptoplast fusion of the fungi. The degree of degradation of the dioxins was a little higher than that by the bioremediation with the fungi. In addition, the ability of fusants for degradation of dioxins decreased in the repeated subcultures.

通过使用二恶英降解真菌及其由真菌的原生质体融合形成的二恶英降解真菌，对土壤中二恶英的生物降解和通过生物修复进行了焚化。而且，还进行了具有较高能力的二恶英降解能力的二恶英的形成。还进行了真菌的融合形成。1。每种二恶英（2、7-DCDD，2、4、8-TCDF和2、3、7、8-TCDD）在土壤中可以通过使用二恶英降解真菌的生物修复来降解。每个二恶英分别降解约70％，65％和60％，持续30天2。在稻田中约有90％的二恶英在用二恶英降解的真菌生物化30天，从而降解。通过生物修复，二恶英在SOLIS中的毒性被ALOS消除。此外，通过在生物修复过程中添加表面活性剂增加了二恶英的降解程度。3。通过与二恶英可降解真菌生物修复降解60天，大约有50％的二恶英在降解。发现灰烬中二恶英的程度大大增加了生物修复。4。稻田中的二恶英可能会因生物修复而与真菌的prpoplast融合形成的融合降解。二恶英的降解程度略高于与真菌的生物修复。另外，在重复的亚文化中，融合剂降解二恶英的能力降低了。