Research and development on purification methods for dioxins, environmental ha-manes and indnerated ashes by use of a minnbial preparations and an enzyme preparations prepared from a fungus capable of degradation of dioxins

利用降解二恶英的真菌制剂和酶制剂净化二恶英、环境哈尼和焚烧灰方法的研究与开发

基本信息

批准号：
18580166
负责人：
TACHIBANA Sanro
金额：
$ 2.39万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

The purification methods for dioxins, environmental hormones, and incinerated ash were tried to develop by use of microbial and enzyme preparations made from a fungus capable of degradation of dioxins, and the following results were obtained.1. The purification method of dioxin- and environmental hormone-contaminated soils by use of the microbial preparations was developed by investigating the added amount of the preparations and treatment time and so on. Eight four percent of the DDT-contaminated soil could be purified by the method for 30 days with 5% of the preparations. And, 35 to 66% of the dioxins (2,8-DCDD and 2,4,8-TCDF)-contaminated soils were purified by the method for 30 days with 2 to 5% of the preparations. Furthermore, the method for enhancement of growth of the fungus on the preparations in soil was also developed by addition of nutrients.2. Enzyme preparations were made from a crude enzyme from the fungus by capsulation. The purification methods of the contaminated soil … More s as well as those waters were developed by using the preparations. Twenty seven and 15% of DDT- and dioxin (TCB)-contaminated soils were purified by the method for 30 days, respectively. And, thirty five and 26% of DIM' and TCB-contaminated waters were also purified by using the preparations for 3 days, respectively. Furthermore, the enzyme activity in the preparations could be stabilized by clarification of the stabilizing factors. The preparations could be reused at least two times when the contaminated waters were purified by the method. The evaluation method for degradation capability of the preparations was also developed.3. The purification method for an incinerated ash was developed by using the microbial preparations with soil having pH buffer capacity and a surfactant enhancing growth of the fungus. About 40% of dioxins (PCDD/Fs) in the ash were purified by the method for 30 days. However, degradation of PCDD/Fs in the ash with the enzyme preparations could not be almost preceded by the inactivation of enzymes for the alkalinity of ash.4. There was no effect of microorganisms originally existed in the soil and dehydrogenase activity related to the activity of microorganisms in soil on the treatment of soil with the microbial and enzyme preparations. Effect of plant cultivation on the soil treatment was not also recognized. Less

利用降解二恶英的微生物和酶制剂，尝试开发了二恶英、环境激素和焚烧灰的净化方法，取得了以下成果： 1．二恶英和环境激素的净化方法。通过调查制剂的添加量和处理时间等，开发了使用微生物制剂处理激素污染的土壤，可以净化百分之八四的DDT污染土壤。用5％的制剂，用该方法处理30天，用2的方法处理30天，净化了35％至66％的二恶英(2,8-DCDD和2,4,8-TCDF)污染的土壤。此外，还开发了通过添加营养物质来促进真菌在土壤中的生长的方法。2．通过使用该制剂开发了污染土壤和水的净化方法，其中 27% 和 15% 的 DDT 和二恶英 (TCB) 污染土壤均通过该方法得到净化。并且，通过使用该制剂3天，分别净化了35％和26％的DIM'和TCB污染的水。此外，还检测了酶活性。通过澄清稳定因子，制剂可重复使用至少两次，并建立了制剂降解能力的评价方法。 3．通过使用具有pH缓冲能力的土壤和促进真菌生长的表面活性剂来开发焚烧灰，通过该方法净化了灰中约40%的二恶英(PCDD/Fs)。 30天后，酶制剂对灰烬中的PCDD/Fs的降解几乎不能先于灰烬的碱度而使酶失活。4．与土壤中微生物的活性有关的微生物和酶制剂对土壤的处理效果也尚未被认识到。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Trails for degradation of DDT-contaminated waste water by an ezyme Preparations from white-rot fungi

白腐真菌制剂降解DDT污染废水的试验

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Satoshi;Somamoto;Keita;Fijii;Kazutaka;Itoh;Sanro;Tachibana
通讯作者：
Tachibana

数種の担体を用いた微生物製剤作製の試み

尝试使用多种类型的载体生产微生物制剂

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
塔村真一郎;宮本康太;石川敦子;井上明生;古山健太
通讯作者：
古山健太

白色腐朽菌からの微生物製剤作製の試み

尝试从白腐真菌中制备微生物制剂

DOI：
发表时间：
2006
期刊：
第51回リグニン討論会講演集
影响因子：
0
作者：
Hiromitsu Kisanuki;Arisa Nakai;Akiko Nadamoto;Masako Wakino;木佐貫博光・中村由実・中井亜理沙・石田清;木佐貫博光・灘本明子・林佐智子・脇野雅子・中井亜理沙;木佐貫博光;木佐貫博光;佐々木良輝
通讯作者：
佐々木良輝

Trials for degradation of dioxins in an incinerated ash by crude enzymes from white-rot fungi

白腐真菌粗酶降解焚烧灰中二恶英的试验

DOI：
发表时间：
2006
期刊：
影响因子：
0
作者：
Kouki;Kurita;Kazutaka;Itoh;Sanro;Tachibana
通讯作者：
Tachibana

ダイオキシン分解菌からの酵素を用いた酵素製剤作製の試み

尝试使用二恶英降解菌的酶生产酶制剂

DOI：
发表时间：
2006
期刊：
第51回リグニン討論会講演集
影响因子：
0
作者：
Hiromitsu Kisanuki;Arisa Nakai;Akiko Nadamoto;Masako Wakino;木佐貫博光・中村由実・中井亜理沙・石田清;木佐貫博光・灘本明子・林佐智子・脇野雅子・中井亜理沙;木佐貫博光;木佐貫博光;佐々木良輝;塔村真一郎;藤井啓太
通讯作者：
藤井啓太