Development of the method for purification of dioxins, environmental hormones and pesticide residue by using immobilized enzyme from fungus capable of degradation dioxins

降解二恶英真菌固定化酶纯化二恶英、环境激素和农药残留方法的研制

基本信息

批准号：
16580136
负责人：
TACHIBANA Sanro
金额：
$ 2.43万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Development of the method for purification of pollution caused by dioxins, environmental hormones, and pesticides residue using immobilized enzyme and crude enzyme from dioxins degradable fungi was tried.1.Effect of degradation rate on concentration of entrapment agents as well as the enzyme activity in immobilized enzyme retained by entrapment was clarified. The method for purification of dioxins, environmental hormones and pesticides residue in soil was developed. Forty five to 80% of TCB, PCDD/Fs, DDT and Lindane were degraded by the method for 30 days. Furthermore, the immobilized enzyme with dual-membrane was also prepared. However, the degradation rate was almost same as that of the immobilized enzyme with single-membrane.2.The method for degradation of dioxins (TCB, PCDD/Fs) by bioreactor using immobilized enzyme as a degradation agent in a short period was developed. The degradation rate of TCB and PCDD/Fs for 72 hours were 65% and 60% (comparison of TEQ value with that of untreated soil), respectively. The degradation rate by the method using bioreactor was five times faster than that by the method without using bioreactor. This method can be applicable to purification of pollution caused by environmental hormones and pesticides residue.3.Since incinerated ash was alkaline and optimum pH (pH 4.5) of the crude enzyme is different from the pH of the ash, it was proven that many enzymes were necessary for the degradation of dioxins, though the degradation was occurred in a low rate (about 30%). Further, dioxins could not almost degraded by using immobilized enzyme for the much inorganic salt in incinerated ash. However, the method for purification of incinerated ash by using dioxins degradable fungus as a degradation agent was developed. Dioxins in incinerated ash were degraded by the method for 30 days (40%, comparison of TEQ value with that of untreated ash).

使用二恶英的二恶英，环境激素和农药残留的污染方法的开发，尝试使用二恶英降解真菌中的固定酶和粗酶。1。阐明了通过夹带保留的酶。开发了二恶英，环境激素和农药残留物的方法。该方法将45至80％的TCB，PCDD/FS，DDT和Lindane降解30天。此外，还制备了用双膜的固定酶。然而，降解速率与单膜固定酶的降解率几乎相同。2。使用固定化酶作为短期内开发了使用固定酶作为降解剂的生物反应器降解二恶英（TCB，PCDD/FS）的方法。 TCB和PCDD/FS的降解率72小时为65％和60％（TEQ值与未处理土壤的值分别比较）。该方法使用生物反应器的降解速率是不使用生物反应器的方法快的速度五倍。该方法可用于纯化由环境激素和农药残留引起的污染。3.CANCENCINEDASH是碱是碱性和最佳的原油pH（pH 4.5），与灰烬的pH值不同，证明许多酶都证明了许多酶尽管降解速率低（约30％），但对于二恶英的降解是必需的。此外，二恶英几乎无法通过在焚化灰分中使用固定的酶作为无机盐的固定酶来降解。但是，开发了通过使用二恶英降解真菌作为降解剂纯化烧焦的方法。该方法将燃烧的灰分中的二恶英降解30天（40％，TEQ值与未经处理的灰分的值进行比较）。

项目成果

期刊论文数量（60）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

白色腐朽菌から抽出酵素によるPolychlorinated Biphenyls (PCBs)分解の試み

尝试使用从白腐真菌中提取的酶来降解多氯联苯 (PCB)

DOI：
发表时间：
2005
期刊：
第55回日本木材学会大会研究発表要旨
影响因子：
0
作者：
SASAKI;K.;S.Miyoshi;松尾保洋;佐々木良輝;松尾保洋
通讯作者：
松尾保洋

Biodegradation of Dioxins with a crude enzyme from white-rot fungi.

用白腐真菌的粗酶生物降解二恶英。

DOI：
发表时间：
2005
期刊：
Proceedings of The 50th Lignin Symposium, Nagoya
影响因子：
0
作者：
SASAKI;K.;S.Miyoshi;松尾保洋;佐々木良輝;松尾保洋;佐々木良輝;小舟瞬;Sinsuke Miyoshi et al.;Yoshihiro Matsuo et al.;Yoshiteru Sasaki et al.
通讯作者：
Yoshiteru Sasaki et al.

Study on degradation of polychlorinated biphenyls with an enzyme from white rot fungi.

白腐真菌酶降解多氯联苯的研究

DOI：
发表时间：
2005
期刊：
Proceedings of The 50th Lignin Symposium, Nagoya
影响因子：
0
作者：
SASAKI;K.;S.Miyoshi;松尾保洋;佐々木良輝;松尾保洋;佐々木良輝;小舟瞬;Sinsuke Miyoshi et al.;Yoshihiro Matsuo et al.
通讯作者：
Yoshihiro Matsuo et al.

Bioremediation of dioxin-contaminated soil with fungi capable of degradation of dioxins.

利用能够降解二恶英的真菌对二恶英污染的土壤进行生物修复。

DOI：
发表时间：
2004
期刊：
Abstracts of the 54th Annual Meeting of The Japan Wood Research Society, Sapporo
影响因子：
0
作者：
SASAKI;K.;S.Miyoshi;松尾保洋;佐々木良輝;松尾保洋;佐々木良輝;小舟瞬;Sinsuke Miyoshi et al.;Yoshihiro Matsuo et al.;Yoshiteru Sasaki et al.;Yoshihiro Matsuo et al.;Yoshiteru Sasaki et al.;Shun Kobune et al.;S.Miyoshi;松尾保洋;松尾保洋;小舟瞬;佐々木良輝;松尾保洋;鷹見純子;木村圭助;木村圭助;Yoshihiro Matsuo et al.;Junko Takami et al.;Keisuke Kimura et al.;Keisuke Kimura et al.
通讯作者：
Keisuke Kimura et al.

ダイオキシン分解菌からの固定化酵素によるPolychlorinatedBiphenyls(PCBs)分解の試み

尝试使用二恶英降解细菌的固定化酶降解多氯联苯 (PCB)

DOI：
发表时间：
2004
期刊：
第54回日本木材学会大会研究発表要旨集
影响因子：
0
作者：
SASAKI;K.;S.Miyoshi;松尾保洋;佐々木良輝;松尾保洋;佐々木良輝;小舟瞬;Sinsuke Miyoshi et al.;Yoshihiro Matsuo et al.;Yoshiteru Sasaki et al.;Yoshihiro Matsuo et al.;Yoshiteru Sasaki et al.;Shun Kobune et al.;S.Miyoshi;松尾保洋;松尾保洋;小舟瞬;佐々木良輝;松尾保洋;鷹見純子;木村圭助;木村圭助;Yoshihiro Matsuo et al.;Junko Takami et al.;Keisuke Kimura et al.;Keisuke Kimura et al.;松尾保洋
通讯作者：
松尾保洋