Study on production of biologically active substances by immobilized cells

固定化细胞生产生物活性物质的研究

基本信息

批准号：
10556039
负责人：
TACHIBANA Sanro
金额：
$ 3.33万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

Production of taxol and ginkgolides by immobilized cells from Kyaraboku (Taxus cuspidata var. nana) and Icho (Ginkgo biloba) was conducted. And, production of taxol by pestalotia fungi isolated from inner bark of Kyaraboku was also conducted.1. Cells induced from Kyaraboku stems were produced taxol by cell suspension cultures. The amount of taxol production was about a half compared to those of the intact plant. However, the amount of the production was increased about 10-holds by addition of an elicitor and a biogenetic precursor to the cell cultures.2. Incubated cells could be immobilized with alaginate. The immobilized cells released taxol into medium. The amount of taxol released into the medium was about 60 to 80% of the taxol produced in the cells. The immobilized cells released continually taxol for 4 weeks. The amount of taxol released into the medium was 0.3 to 0.6mg/L.A possiblity for production of taxol by use of a bioreactor was recognized.3. Some pestalotia fungi were isolated from inner bark of Kyaraboku. The fungi could be produced taxanes including taxol, however, the amount of taxol production are under investigation.4. Cells from Ginkgo seeds were produced ginkgolides by cell suspension cultures. The amount of ginkgolides production was almost same as those of the intact plant. The amount of ginkgolides production was increased about 5 times by addition of an elicitor and a biogenetic precursor to the cultures.5. Incubated cells could be immobilized with alaginate. The immobilized cells released ginkgolides into time medium. The amount of ginkgolides released into the medium was about 20% of ginkgolides produced in the cells. A possiblity for production of taxol by use of a bioreactor was recognized.

利用来自 Kyaraboku (Taxus cuspidata var. nana) 和 Icho (Ginkgo biloba) 的固定化细胞生产紫杉醇和银杏内酯。并且，还利用从Kyaraboku内皮中分离得到的盘斑真菌进行了紫杉醇的生产。 1．由Kyaraboku茎诱导的细胞通过细胞悬浮培养产生紫杉醇。与完整植物相比，紫杉醇的产量约为一半。然而，通过向细胞培养物中添加诱导子和生物遗传前体，产量增加了约10个保持量。2．孵育的细胞可以用藻酸盐固定。固定的细胞将紫杉醇释放到培养基中。释放到培养基中的紫杉醇量约为细胞中产生的紫杉醇的60%至80%。固定化细胞持续释放紫杉醇4周。释放到培养基中的紫杉醇量为0.3～0.6mg/L。认识到利用生物反应器生产紫杉醇的可能性。 3．从Kyaraboku 的内皮中分离出一些盘菌。真菌可产生包括紫杉醇在内的紫杉烷类化合物，但紫杉醇的产量尚在调查中。 4．银杏种子细胞通过细胞悬浮培养产生银杏内酯。银杏内酯的产量与完整植株几乎相同。通过向培养物中添加诱导子和生物遗传前体，银杏内酯的产量增加了约5倍。5．孵育的细胞可以用藻酸盐固定。固定化细胞将银杏内酯释放到时间培养基中。释放到培养基中的银杏内酯量约为细胞中产生的银杏内酯量的20%。认识到通过使用生物反应器生产紫杉醇的可能性。