Study on utilization of wood extractives from Kyaraboku (Taxus cuspidata var.nana) belonging to trees of genus taxus

红豆杉属木材提取物的利用研究

• 批准号: 07556044
• 负责人: TACHIBANA Sanro
• 金额: $ 3.07万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556044/
• 关键词: Tissue cultures; Elicitor; Taxus cuspidata var.nana; Taxol; Anti-biotic activity; Callus cultures; Chitoheptaose; Cell suspension cultures; 固定化細胞; キトオリゴ糖; 生合成前駆体; 微生物変換; ジベレリン

Utilization of wood extractives from Kyaraboku were examined.1.Taxol, a strong anti-tumor agent, in T.cuspidata was produced by callus cultures of Kyaraboku. Amount of production of taxol was increased 5.1 times larger than that of the control (no addition) when chito-oligosaccharides as a elicitor for ehhancement of taxol production were added to the calli. Chitoheptaose was found to be the best elicitor in the chito-oligosaccharides.2.Taxol was produced by cell suspension cultures of Kyaraboku. Taxol production increased 6.3 times larger than that of the control when chitoheptaose and jasmonic acid were added to the suspension cultures.3.Taxol was released into the medium during incubation when cultured cells were incorporated with sodium alginate and incubated. The maximum amount of taxol released into the medium was 0.6 mg/L.4.Taxinine in Kyaraboku and its derivatives have anti-biotic activities against Cochiliobolus miyabeanus, Altenaria kikuchiana, Colletotrichum fragariae, Botryisis cinerea, and Corynespora cassiicola.5.A trial of preparation of taxol mimic by condensation of the BaccatinIII like compound derived from taxinine and phenylisosserin was conducted. Phenylisoserin, the side chain of taxol, was stereoselectively synthesized from benzaldehyde in a total yield of 0.9%. However, preparation of the BaccationIII like compound from taxinine are now being conducted.

检查了从kyaraboku的木材提取物的利用。1.Taxol，一种强的抗肿瘤剂，在T. cuspidata中是由Kyaraboku的愈伤组织培养物产生的。当将Chito-Oligosacachirides作为Ehhancement的Ehhancetor添加到Calli中时，紫杉醇的生产量是对照组的5.1倍（无添加）。发现壳糖糖是Chito-Oligosacachilides中的最佳诱因。2.Taxol是由Kyaraboku的细胞悬浮培养物产生的。当将壳糖糖和茉莉酸加入悬浮培养物中时，紫杉醇产量比对照大的6.3倍。3.Taxol在孵育过程中释放到培养基中，当时将培养的细胞与藻酸钠掺入并孵育。释放到培养基中的最大紫杉醇量为0.6 mg/l.4.甲状腺素及其衍生物具有针对宫核的抗生素活性，基克丘亚纳（Altenaria kikuchiana进行了源自类动物素和苯舍蛋白的baccatiniii类化合物。苯共糖蛋白是紫杉醇的侧链，立体选择性从苯甲醛合成，总产率为0.9％。但是，现在正在进行制备BACCATIONIII类化合物的化合物。

项目成果

上野 淳一郎: "イチイ科樹木の抽出成分(VI)イチイ葉の抽出成分について" 第46回日本木材学会大会研究発表要旨集. 407-407 (1996)

Junichiro Ueno：“红豆杉树的提取成分（六）关于红豆杉叶的提取成分”第46届日本木材学会会议摘要407-407（1996）。

K.Kurose: "Extractives of Taxus cuspidata Sieb.et.Zucc.Leaves and chemoeaxonomy of Trees of Genus Taxus" Bull.Ehime University Forest. 第33号. 1-16 (1995)

K.Kurose：“红豆杉提取物 Sieb.et.Zucc.Leaves 和红豆杉属树木的化学分类学”Bull.Ehime University Forest No. 33. 1-16 (1995)

Katsuhiko Kurose et al.: "Extractives of Taxus cuspidata Sieb.et Zucc. Leaves and Chemotaxonomy of Trees of Genus Taxus." Bull.Ehime Univ.Forest. No.33. 1-16 (1995)

Katsuhiko Kurose 等人：“红豆杉 Sieb.et Zucc 的提取物。红豆杉属树木的叶子和化学分类学”。

吉田 真弓: "イチイ科樹木の組織培養による抗ガン性物質,タキソ-ルの生産の試み" 第46回日本木材学会大会研究発表要旨集. 7-7 (1996)

Mayumi Yoshida：“尝试通过红豆杉树的组织培养来生产紫杉醇，一种抗癌物质”第 46 届日本木材学会会议上的研究报告摘要 7-7（1996 年）。

上野淳一郎: "イチイ科樹木の抽出成分(VI)-イチイ葉の抽出成分について-" 第46回日本木材学会大会研究発表要旨集. 407-407 (1996)

Junichiro Ueno：“红豆杉树的提取成分（VI）-关于红豆杉叶的提取成分-”第46届日本木材学会会议上的研究报告摘要集407-407（1996年）。