Study on endogenous gibberellins in trees and utilization of gibberellins produced by microbial conversion of kaurene

树木内源赤霉素及其微生物转化贝壳杉烯产生赤霉素的研究

基本信息

批准号：
08660208
负责人：
TACHIBANA Sanro
金额：
$ 1.34万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

Gibberellins in sprouts of Kyaraboku (Taxus cuspidata var.nana), formation of fusants by protoplasts fusion of Gibberella fujikuroi, and the effect of additon of gibberellins produced by a microbial conversion with G.fujikuroi on elongation in Kyaraboku and Inugaya (Cephalotaxus harringtonia) sprouts were examined.1. Gibberellin-A3 (GA3), GA4, and a glucosylester of gibberellin were isolated from Kyaraboku sprouts.2. Three fusants (F1, F2, and F3) were formed by protoplasts fusion between G.fujikuroi IFO 9977 and IFO 30337. Each fusant produced GA3, iso-GA3, GA4, GA7, and iso-GA7 by the microbial conversion of (-) -kaurene, as parents fungi produced the same gibberellins by the microbial conversion. However, amounts of production of gibberellins by F2 and F3 fusants were about 1.5 times larger than those of parents fungi.3. Kyaraboku and Inugaya sprouts were elongated about 1.2 times and about 1.1 times longer than those of the control (no addition of the gibberellins produced by the microbial conversion), respectively, when the gibberellins were added to Kyaraboku and Inugaya stems. Furthermore, the content of taxol, a strong anti-tumor agent, in Kyaraboku sprouts was increased about 1.1 times larger than that of the control (no addition of the gibberellins). The contents of harringtonine aklaloids (an anti-tumor agent), especially harringtonine and isoharringtonine, in Inugaya sprouts were increased about 1.2 times larger than that of the control (no addition of the gibberellins)

Gibberellins in sprouts of Kyaraboku (Taxus cuspidata var.nana), formation of fusants by protoplasts fusion of Gibberella fujikuroi, and the effect of additon of gibberellins produced by a microbial conversion with G.fujikuroi on elongation in Kyaraboku and Inugaya (Cephalotaxus harringtonia)检查了芽。1。从kyaraboku Sprouts.2。 Three fusants (F1, F2, and F3) were formed by protoplasts fusion between G.fujikuroi IFO 9977 and IFO 30337. Each fusant produced GA3, iso-GA3, GA4, GA7, and iso-GA7 by the microbial conversion of (-) -kaurene, as parents fungi produced the same gibberellins by the microbial conversion.但是，F2和F3弹药的生产量量是父母真菌3的1.5倍。当kyaraboku和inugaya芽芽素的拉长约1.2倍和约1.1倍，比对照组长（不添加微生物转化率产生的gibberellins），当时gyaraboku添加到kyaraboku和inugaya茎时。此外，在Kyaraboku芽中，紫杉醇（一种强大的抗肿瘤剂）的含量增加了约1.1倍的对照（不添加Gibberellins）。在Inugaya芽孢杆菌中，Harringtonine Aklaloid（抗肿瘤剂）的含量（抗肿瘤剂），尤其是Harringtonine和Isoharringtonine，在Inugaya芽中的含量比对照组大1.2倍（不添加gibberellins）。