Degradation and purification of dioxins by bioremediation with fungi having ability for degradation of dioxins

利用具有二恶英降解能力的真菌进行生物修复对二恶英的降解和纯化

基本信息

批准号：
11694214
负责人：
TACHIBANA Sanro
金额：
$ 4.35万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

Screening of fungi having ability for degradation of dioxins from the nature was conducted in temperate and tropical zone, and selected several fungi. Degradation of dioxins in soils, incinerated ashes and water by bioremediation with the selected fungi was conducted. And, furthermore, formation of fusants having higher ability for degradation of dioxins was conducted by protoplast fusion between the two selected fungi.1. Dioxins (2,7-DCDD, 2,8-DCDD, 2,4,8-TCDF, 2,3,7,8-TCDD, 2,3,4,7,8-PCDF and OCDD) were degraded by the selected fungi from the nature in a liquid medium. The degree of degradation of each dioxin was about 95%, 85%, 70%, 60%, 95% and 40%, respectively for 30 days incubation.2. Biodegradation of dioxins (2,7-DCDD, 2,4,8-TCDF and 2,3,7,8-TCDD) in soils could be degraded by bioremediation with the selected fungi. Each dioxin was degraded about 70%, 60% and 50%, respectively for 30 days.3. About 70% of dioxins in the soils (contamination level: about 1300pg-TEQ/g) was degraded by bioremediation with the selected fungi for 30 days. About 70% of the toxicity of dioxins in the soils was also removed by the bioremediation. And furthermore, the degree of degradation of the dioxins was increased about 10% by addition of a surfactant when the bioremediation was conducted.4. About 65% of dioxins in incinerated ashes was degraded by bioremediation with the selected fungi for 60 days. It was found that the degree of degradation of dioxins in the ashes could be almost completely degraded by the repeated treatment of the bioremediation.5. Dioxins in aqueous environment could be degraded considerably by bioremediation with the selected fungi after adsorption of dioxins by activated carbons and mixing of non-polluted soils with the adsorbed carbons. It was found that dioxins in water were almost completely adsorbed on to activated carbons.

在温带和热带地区进行了具有从自然界降解的真菌筛查，并选择了几种真菌。通过与选定的真菌进行生物修复，在土壤中降解二恶英，骨灰和水。此外，通过两种选定的真菌之间的原生质体融合来进行具有更高降解能力的融合者的形成。二恶英（2,7-DCDD，2,8-DCDD，2,4,8-TCDF，2,3,7,8-TCDD，2,3,4,7,8-PCDF和OCDD）被选定的真菌从液体培养基中的性质中降低。每种二恶英的降解程度分别约为95％，85％，70％，60％，95％和40％，孵育30天2。土壤中二恶英（2,7-DCDD，2,4,8-TCDF和2,3,7,8-TCDD）的生物降解可以通过与选定的真菌进行生物修复来降解。每个二恶英分别降解约70％，60％和50％，持续30天3。土壤中约70％的二恶英（污染水平：约1300pg-Teq/g）通过与所选真菌生物修复降解30天。生物修复也消除了土壤中二恶英的毒性的70％。此外，在进行生物修复时，通过添加表面活性剂增加了二恶英的降解程度。4。通过与选定的真菌生物修复降解65％的二恶英大约65％的二恶英60天。已经发现，重复对生物修复的重复处理几乎可以完全降解灰烬中二恶英的降解程度。5。通过活化的碳吸附二恶英并将非污染土壤与吸附的碳混合在一起，可以通过与所选真菌进行生物修复来大大降解二恶英。发现水中的二恶英几乎完全吸附到活性碳中。