Degradation and purification of dioxins by bioremediation with fungi having ability for degradation of dioxins

利用具有二恶英降解能力的真菌进行生物修复对二恶英的降解和纯化

基本信息

批准号：
11694214
负责人：
TACHIBANA Sanro
金额：
$ 4.35万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

Screening of fungi having ability for degradation of dioxins from the nature was conducted in temperate and tropical zone, and selected several fungi. Degradation of dioxins in soils, incinerated ashes and water by bioremediation with the selected fungi was conducted. And, furthermore, formation of fusants having higher ability for degradation of dioxins was conducted by protoplast fusion between the two selected fungi.1. Dioxins (2,7-DCDD, 2,8-DCDD, 2,4,8-TCDF, 2,3,7,8-TCDD, 2,3,4,7,8-PCDF and OCDD) were degraded by the selected fungi from the nature in a liquid medium. The degree of degradation of each dioxin was about 95%, 85%, 70%, 60%, 95% and 40%, respectively for 30 days incubation.2. Biodegradation of dioxins (2,7-DCDD, 2,4,8-TCDF and 2,3,7,8-TCDD) in soils could be degraded by bioremediation with the selected fungi. Each dioxin was degraded about 70%, 60% and 50%, respectively for 30 days.3. About 70% of dioxins in the soils (contamination level: about 1300pg-TEQ/g) was degraded by bioremediation with the selected fungi for 30 days. About 70% of the toxicity of dioxins in the soils was also removed by the bioremediation. And furthermore, the degree of degradation of the dioxins was increased about 10% by addition of a surfactant when the bioremediation was conducted.4. About 65% of dioxins in incinerated ashes was degraded by bioremediation with the selected fungi for 60 days. It was found that the degree of degradation of dioxins in the ashes could be almost completely degraded by the repeated treatment of the bioremediation.5. Dioxins in aqueous environment could be degraded considerably by bioremediation with the selected fungi after adsorption of dioxins by activated carbons and mixing of non-polluted soils with the adsorbed carbons. It was found that dioxins in water were almost completely adsorbed on to activated carbons.

在温带和热带地区对自然界中具有降解二恶英能力的真菌进行了筛选，筛选出几种真菌。利用选定的真菌进行生物修复，降解土壤、焚烧灰烬和水中的二恶英。此外，通过两种选定真菌之间的原生质体融合，形成了具有较高二恶英降解能力的融合体。 1．二恶英（2,7-DCDD、2,8-DCDD、2,4,8-TCDF、2,3,7,8-TCDD、2,3,4,7,8-PCDF 和 OCDD）通过在液体培养基中从自然界中选择真菌。培养30天，每种二恶英的降解程度分别约为95%、85%、70%、60%、95%和40%。2．土壤中二恶英（2,7-DCDD、2,4,8-TCDF 和 2,3,7,8-TCDD）的生物降解可以通过所选真菌的生物修复来降解。 30天内，每种二恶英分别降解约70%、60%和50%。3．通过所选真菌的生物修复30天，土壤中约70%的二恶英（污染水平：约1300pg-TEQ/g）被降解。土壤中约70%的二恶英毒性也通过生物修复被去除。而且，生物修复过程中添加表面活性剂后，二恶英的降解程度提高了约10%。 4．通过使用选定的真菌进行 60 天的生物修复，焚烧灰烬中约 65% 的二恶英被降解。结果发现，通过生物修复的反复处理，灰烬中二恶英的降解程度几乎可以完全降解。5．在活性炭吸附二恶英并将未污染的土壤与吸附的碳混合后，通过使用选定的真菌进行生物修复，可以显着降解水环境中的二恶英。结果发现，水中的二恶英几乎完全被活性炭吸附。