Study on biotechnological production of biologically active substances from extracts of Japanese taxus trees.

日本红豆杉提取物生物技术生产生物活性物质的研究。

基本信息

批准号：
04660190
负责人：
TACHIBANA Sanro
金额：
$ 1.09万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660190/
关键词：
Japanese taxus trees Kyaraboku Tissue culture Enzymatic conversion Taxol Taxine Elicitor

项目摘要

The aim of the study was biotechnological production of biologically active substances from extracts of Kyaraboku(Taxus cuspidata var.nana Rehd).1. Extracts in leaves and bark of kyaraboku.Two bisflavones (sciadopitysin and ginkgetin), two flavonols (isorhamnetin and quercetin), and two taxane-type of diterpenoids (taxinine and taxinine B) were isolated from the leaves of Kyaraboku. It was considered that taxinine could be converted to taxol mimic having anti-carcenogenic activity by enzymatic conversion with lipase.From the bark of Kyaraboku, taxol having strong anti-carcenogenic activity, was isolated in the yield of 0.001% to dry bark. The content of taxol in the bark was very small, however, it was considered that content of taxol could be increased by tissue culture of Kyaraboku.2.Pruduction of taxol mimic by enzymatic conversion of taxinine with lipase.Taxol mimic can be produced by reduction of enone carbonyl group in taxinine and followed by esterfication with lipase. Taxol mimic's side chain, phenylisoserine was synthesized from E-cinnamic acid through 8 steps in the total yield of 15%. Reduction of the carbonyl group with di-butylaluminium hydride are now being conducted.3. Production of taxol by tissue culture of Kyaraboku.Taxol was found to be produced in the calli induced from Kyaraboku stems and leaves. The amount of taxol in the calli was 1.1 times higher than that in intact plant. To increase the amount of taxol, the calli was eliciated with chito-oligosaccharides in various concentrations. The amount of taxol was rised up to 10 times higher than that of untreated calli.

该研究的目的是从kyaraboku提取物（taxus cuspidata var.nana rehd）提取物生物学活性物质的生物技术产生。1。 kyaraboku.two Bisflavones（Sciadopitysin和Ginkgetin）的叶子和树皮中的提取物，两个黄酮醇（Isorhamnetin和quercetin），以及两个二萜类甲苯二甲酸酯型（紫杉胺和紫杉胺B）（紫杉胺和紫杉氨酸B）。据认为，可以通过酶的脂肪酶转化具有抗癌活性的紫杉胺模仿。但是，树皮中紫杉醇的含量很小，但是，认为紫杉醇的含量可以通过kyaraboku的组织培养来增加。2。通过酶促的脂肪胺与脂肪酶的酶促转化，可以通过减少来产生紫杉醇模仿。 eNONE羰基的特拉苯胺，然后用脂肪酶进行酯进行酯。紫杉醇模仿的侧链，苯共苷烯是从电子甲酸合成的，总产量为15％的8个步骤。现在正在进行氢化氢甲酰胺的羰基还原。3。通过kyaraboku的组织培养生产紫杉醇。发现在kyaraboku茎和叶子引起的calli中生产taxol。 calli中紫杉醇的数量是完整植物中的1.1倍。为了增加紫杉醇的量，calli是通过各种浓度的奇托 - 寡糖引起的。紫杉醇的数量的升高是未经处理的Calli的10倍。