Diversity Supplement for Updating the Mixture in Mechanisms of Phthalate Toxicity in the Ovary

用于更新卵巢邻苯二甲酸盐毒性机制混合物的多样性补充

基本信息

批准号：
10818882
负责人：
Genoa R Warner
金额：
$ 7.42万
依托单位：
NEW JERSEY INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-05-05 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10818882
关键词：
Acceleration Affinity Applications Grants Behavior Biochemical Biological Biology Cell Culture Techniques Cell membrane Cells Cessation of life Chemical Structure Chemicals Chemistry Child Complex Culture Media DNA-Binding Proteins Data Development Dioxins Disease Endocrine Disruptors Endocrine disruption Environment Environmental Health Environmental Science Enzymes Equipment Esters Exposure to Family Female Female infertility Funding Gene Expression General Population Genes Genetic Transcription Genomics Hormones Human Hydrocarbons Illinois Image In Vitro Industrialization Infertility Label Laboratories Learning Length Mass Spectrum Analysis Massive Parallel Sequencing Mediating Medical Mentors Mentorship Metabolism Methods Modernization Molecular Nature New Jersey Oocytes Ovarian Ovarian Follicle Ovarian Tissue Ovarian aging Ovary Phthalic Acids Plasticizers Population Postbaccalaureate Premature Ovarian Failure Prevalence Prevention Property Proteins Public Health Reproduction Research Research Personnel Research Training Sampling Side Solvents Structure Techniques Technology Testing Tissues Toxic effect Toxicology Toy Training Universities Update Urine Visualization Woman building materials career career development cell type chromatin immunoprecipitation consumer product environmental chemical exposure exposed human population hands on research imaging modality innovation insight lipophilicity men mouse model next generation sequencing novel ovotoxicity parent grant parent project personal care products phthalates post-doctoral training professor receptor reproductive reproductive organ reproductive system disorder screening skills small molecule toxicant transcription factor

项目摘要

PROJECT SUMMARY/ABSTRACT This is a proposal for a Diversity Supplement to fund hands-on research training and career development for a promising research technician receiving post-baccalaureate training at the New Jersey Institute of Technology. The parent grant for this proposal is R00ES031150 to facilitate Dr. Genoa Warner’s transition to an independent research career as an Assistant Professor in the Department of Chemistry and Environmental Science at the New Jersey Institute of Technology, where she draws from her diverse scientific background in chemistry and biology to investigate important research questions in environmental health. Dr. Warner has extensive training in endocrine disruption and sustainable chemistry and has completed postdoctoral training in reproductive toxicology at the University of Illinois under the mentorship of Dr. Jodi A. Flaws, where she has learned technical and profession skills necessary for her to become a successful independent investigator and leader in the field of reproductive toxicology. In her new laboratory, Dr. Warner investigates the mechanisms by which phthalates, a group of endocrine disrupting chemicals with ubiquitous human exposure, cause ovarian toxicity. Further, she investigates the metabolism of phthalates by ovarian tissue because phthalate metabolites mediate phthalate- inducted toxic effects, contributing to infertility and premature ovarian failure. In the prosed studies, Dr. Warner will mentor the Diversity Supplement candidate, Stephanie Martinez, in pursuing research within the scope of the original project aims to craft her own research niche. Specific Aim 1 of the parent project aims to identify how phthalates partition into ovarian tissue during culture via mass spectrometry and imaging methods using chemically modified phthalates. Specific Aim 2 will determine how phthalate toxicity varies between cell types of the ovary using next generation sequencing techniques. Finally, Specific Aim 3 will identify the genomic localization of phthalates in the ovary by adapting the biochemical technique of chemical affinity capture paired with massively parallel DNA sequencing (Chem-seq) to provide information on the genes and proteins targeted by phthalates. Overall, the proposed studies will provide novel chemical and mechanistic insight into causes of phthalate-induced toxicity in the ovary that contribute to female infertility and premature ovarian failure. The methods combine chemical and biological techniques to develop a new, widely applicable technology for investigating the mechanisms of endocrine disrupting chemicals.

项目概要/摘要这是一项多样性补充提案，旨在资助实践研究培训和职业发展有前途的研究技术人员在新泽西理工学院接受学士后培训。该提案的家长补助金是 R00ES031150，以促进 Genoa Warner 博士过渡到独立在化学与环境科学系担任助理教授的研究生涯新泽西理工学院，她从化学和化学领域的多元化科学背景中汲取灵感华纳博士接受过广泛的培训，以研究环境健康方面的重要研究问题。内分泌干扰和可持续化学领域的研究人员，并已完成生殖领域的博士后培训在 Jodi A. Flaws 博士的指导下，她在伊利诺伊大学毒理学专业学习了技术以及成为该领域成功的独立调查员和领导者所需的专业技能华纳博士在她的新实验室中研究了邻苯二甲酸盐的机制，人类普遍接触的一组内分泌干扰化学物质会导致卵巢毒性。研究卵巢组织对邻苯二甲酸盐的代谢，因为邻苯二甲酸盐代谢物介导邻苯二甲酸盐- 华纳博士在进行的研究中指出，会引起毒性作用，导致不孕和卵巢早衰。将指导多样性补充候选人斯蒂芬妮·马丁内斯 (Stephanie Martinez) 在以下范围内进行研究最初的项目旨在打造自己的研究利基，父项目的具体目标 1 旨在确定如何进行。邻苯二甲酸盐在培养过程中通过质谱和成像方法分配到卵巢组织中具体目标 2 将确定邻苯二甲酸盐毒性在细胞类型之间的差异。最后，特定目标 3 将鉴定基因组。通过采用化学亲和捕获配对的生化技术将邻苯二甲酸盐定位在卵巢中通过大规模并行 DNA 测序 (Chem-seq) 提供有关目标基因和蛋白质的信息总体而言，拟议的研究将为解决邻苯二甲酸盐的原因提供新的化学和机制见解。邻苯二甲酸盐引起的卵巢毒性会导致女性不孕和卵巢早衰。方法结合化学和生物技术来开发一种新的、广泛适用的技术研究内分泌干扰化学物质的机制。