CORE--GENETIC MONITORING

核心——基因监测

• 批准号: 7310507
• 负责人: Robert C Young
• 金额: $ 7.8万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7310507
• 关键词: animal colony; biomedical facility; genetic screening; genetic strain; genetically modified animals; inbreeding; laboratory mouse; neoplasm /cancer

This rapidly growing Genetic Monitoring Facility is used by 20 peer-reviewed, funded investigators from 8 Research Programs using animal models in 36 research protocols. In 2003, 97.7% of the research projects utilizing this Facility were supported by peer-reviewed funding. Good genetic monitoring has become critical to the laboratory animal needs of the Fox Chase Cancer Center (FCCC) research community, as animals used in today's biomedical research must not only be free of disease, but also be well defined in terms of their genetic makeup. At present, we monitor 11 different loci located on 9 different chromosomes. We are currently maintaining 170 lines of mice for FCCC investigators and continue to add an average of one new transgenic line per month. During the past year, the Facility has performed 5,856 genetic profile tests, a 225% increase from the 1,800 performed in 1999. Service hours for genetic monitoring services have also increased dramatically, up 140% from 342 in 1999 to 821 in 2003. The daily operation of breed ng colonies is handled by personnel trained and experienced in genetic management and record-keeping. Regular review, advice and guidance is provided by Bruce Elder, Ph.D., a consultant geneticist added in March 2004 who makes quarterly visits and reviews reports and activities in all rodent colonies, and all of the breeding, production and genetic monitoring records have been computerized. These computerized records enable us to closely monitor breeding performance, pedigree lines, and utilization of the various inbred lines and strains. The ability to monitor the genome, and maintain the breeding colonies, of unique immunodeficient and transgenic mice enhances the interaction among Members of the Immunobiology Program as well as Members of the Cellular and Developmental Biology (Burch), Mintz (Tumor Cell Biology), Human Genetics (Testa, Program Leader), Developmental Therapeutics (Kruh, Smith, Weiner, Program Leader), and Cancer Prevention and Control (Clapper) Programs. Genetic Monitoring services have become a critical part of research with animals, and the need for services is expected to continue to increase 18% per year in the future. We propose adding one FTE to support this increase in Facility activities.

20种同行评审，资助的迅速增长的遗传监测设施使用 在36个研究方案中使用动物模型的8个研究计划的研究人员。 2003年， 使用该设施的研究项目中有97.7％得到了经过同行评审的资金的支持。 良好的遗传监测对Fox Chase的实验室动物需求至关重要 癌症中心（FCCC）研究界，因为当今的生物医学研究中使用的动物不仅必须没有疾病，而且还可以很好地定义其基因组成。目前，我们监视位于9个不同染色体上的11个不同基因座。我们目前正在为FCCC调查人员维护170条小鼠，并每月平均增加一条新的转基因线。在过去的一年中，该设施进行了5,856次遗传概况测试，比1999年的1,800个增长了225％。遗传监测服务的服务时间也急剧增加，从1999年的342％到2003年的342％增长了140％。品种NG Colonies的日常运营受到了人事培训和经验丰富的遗传和经验丰富的遗传和记录。定期审查，建议和指导由2004年3月的顾问遗传学家布鲁斯·埃尔德（Bruce Elder）博士提供，他在所有啮齿动物殖民地进行了季度访问和审查报告和活动，所有育种，生产和遗传监测记录都已计算机化。这些计算机记录使我们能够密切监测各种近交和应变的育种性能，谱系线和利用。 The ability to monitor the genome, and maintain the breeding colonies, of unique immunodeficient and transgenic mice enhances the interaction among Members of the Immunobiology Program as well as Members of the Cellular and Developmental Biology (Burch), Mintz (Tumor Cell Biology), Human Genetics (Testa, Program Leader), Developmental Therapeutics (Kruh, Smith, Weiner, Program Leader), and Cancer Prevention and Control (Clapper)程序。遗传监测服务已成为动物研究的关键部分，预计将来对服务的需求将继续增加18％。我们建议添加一个FTE来支持这一设施活动的增加。