CORE--DNA MICROARRAY FACILITY

核心——DNA微阵列设备

• 批准号: 7142491
• 负责人: Robert C Young
• 金额: $ 11.82万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-10 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7142491
• 关键词: DNA; biomedical facility; microarray technology; neoplasm /cancer

Microarray technology has broad applications in cancer research such as identification of cancer markers and gene expression profiling for diagnosis, treatment, and prognosis. Maintenance of a Core DNA Microarray Facility in a comprehensive cancer research center is critical for the success of a growing number of investigators with peer-reviewed funding. The Microarray Facility was established in 1999 using institutional and developmental funds from the CCSG. The Facility provides both printed cDNA and oligo arrays, for investigators to perform gene expression analysis, and full microarray services from RNA sample labeling to array scanning, for those investigators who do not have the capacity to perform microarray experiments in their own laboratories. The Facility also helps investigators to design and print custom microarrays to meet their specific research goals. The Facility is used by 20 investigators, all with peer-reviewed funding, from 9 research Programs in all three Divisions of Fox Chase Cancer Center (FCCC). The microarray usage has increased more than 200% from the year 2002 to 2003 and reached 1,300 microarrays per year. With an increasing number of research grants involving microarray analysis awarded recently, and the establishment of a Laser Capture Microdissection Facility at the Center, combined with RNA amplification procedure which enables small clinical cancer samples to be applied to microarray analysis, we anticipate a 100%-200% increase over the next five years. The microarray products of the Facility include human 40k cDNA and 30k oligo arrays, mouse 15k cDNA and 32k oligo arrays, and 6k yeast oligo arrays. A rat oligo library will be acquired to meet the needs of those investigators using rat models to study breast and ovarian oncogenesis. The Facility has also designed and produced customer design specific arrays, such as DNA repair process-related gene arrays. Standard microarray procedures and optimized labeling and hybridization kits, as well as a Microarray Training Program, have been made available to microarray users. A comprehensive microarray quality control procedure has been implemented to ensure the quality of microarray products and services. The Facility coordinates with the Bioinformatics Facility for microarray data analysis and management.

微阵列技术在癌症研究中广泛应用 癌症标志物和基因表达分析，用于诊断，治疗和预后。 维护核心DNA微阵列在一个综合癌症研究中心的维护是 对于越来越多的调查人员和同行评审的资金成功的成功至关重要。这 微阵列设施于1999年建立了使用机构和发展基金 CCSG。该设施提供了印刷的cDNA和寡量阵列，供研究人员进行基因表达分析，以及从RNA样品标记到阵列扫描的完整微阵列服务，对于那些没有能力在自己的实验室中进行微阵列实验的研究者。该设施还可以帮助调查人员设计和打印自定义微阵列，以实现其特定的研究目标。该设施由20名调查人员使用，所有研究人员都来自同行评审的资金，来自Fox Chase Cancer Center（FCCC）所有三个部门的9个研究计划。从2002年到2003年，微阵列的使用率增长了200％以上，每年达到1,300个微阵列。随着涉及最近授予的微阵列分析的越来越多的研究赠款，并在中心建立了激光捕获的微型分解设施，并结合RNA扩增程序，使小型临床癌症样品可用于微阵列分析，我们预计未来五年将增加100％-200％。该设施的微阵列产品包括人40k cDNA和30K寡核武器阵列，小鼠15K cDNA和32K寡核阵列以及6K酵母寡核阵列。将获得大鼠寡核库，以满足这些研究人员的需求，使用大鼠模型研究乳腺癌和卵巢肿瘤。该设施还设计和生产了特定于客户设计的阵列，例如与DNA维修过程相关的基因阵列。标准的微阵列程序以及优化的标签和杂交套件以及微阵列培训计划已为微阵列用户提供。已经实施了全面的微阵列质量控制程序，以确保微阵列产品和服务的质量。该设施与用于微阵列数据分析和管理的生物信息学设施协调。