CORE--LAB ANIMAL HEALTH

核心--实验动物健康

• 批准号: 7141894
• 负责人: Robert C Young
• 金额: $ 12.42万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-10 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7141894
• 关键词: Rodentias; animal colony; biomedical facility; germ free condition; neoplasm /cancer; veterinary medicine

The Laboratory Animal Health Facility (LAHF) supports an ever-increasing demand for the use of animals in the Fox Chase Cancer Center's (FCCC) Laboratory Animal Facility (LAF). In 2003, the LAHF supported 46 investigators in all 11 research Programs from all three Divisions at the Center. The vast majority (96%) of use was for peer-reviewed, funded research. The increase in mouse usage during the past five years was 116%, and the use of rodents is anticipated to expand approximately 100% in the next five years. Even as importation of mice from collaborators has increased by over 50% in the past five years, the Facility has maintained and improved the health of the colony. All new strains imported from other Facilities are held in quarantine and monitored for murine disease by serology and polymerase chain reaction (PCR) techniques; most have to be rederived by germ-free cesarean or embryo transfer and raised on disease-free foster mothers in microisolator units before permitted into the main research Facility. Over 81 such cesarean rederivations were done in 2003. The health of animals and their freedom from murine pathogens is constantly monitored by sentinel animals in each animal room, and the entire research Facility has remained viral antibody-free since 1998, Severe immune-deficient mice are bred and successfully live up to two years in the FCCC research colony. Pneumocystis, Pasteurella and Helicobacter have already been eliminated from all breeding and immunodeficient rodent areas, and continued systematic rederivation combined with increased microisolator housing is expected to completely eliminate these agents from all FCCC colonies within two years. In 2003, the LAHF performed 11,967 tests (5,600 microbiological cultures, 3,179 ELISA assays, 2,088 (PCR) assays, and 1,100 necropsies and the number of service hours of direct support to research investigators has increased 210% (1,912 in 2003). Technical support includes animal surgery, antibody production in rabbits, mice and rats, collecting samples from animals, tumor line maintenance, necropsies, tumor and cell culture screening, and microbiological/serological testing. The need for technical services has increased almost four-fold from 1998 (616 service hours) to 2003 (1,912 service hours). This increase is expected to continue parallel to the increase in animal numbers, and an increase of one FTE is projected.

实验室动物保健设施（LAHF）支持对使用的不断增长的需求 Fox Chase癌症中心（FCCC）实验室动物设施（LAF）中的动物。 2003年， LAHF在所有三个部门的11个研究计划中支持了46位调查人员 中心。绝大多数使用（96％）用于同行评审，资助的研究。在过去五年中，鼠标使用率的增加为116％，预计啮齿动物的使用将会 在未来五年内扩大约100％。即使在过去五年中，从合作者进口小鼠也增加了50％以上，该设施维持并改善了殖民地的健康状况。从其他设施中进口的所有新菌株均在隔离区中持有，并通过血清学和聚合酶链反应（PCR）技术对鼠类疾病进行监测。大多数人必须通过无菌剖宫产或胚胎转移重新修饰，并在无疾病的母亲中饲养微分散剂单元，然后才能进入主要研究设施。 2003年进行了超过81种此类剖宫产。动物的健康及其免受鼠病原体的自由受到每个动物室中的前哨动物的不断监测，并且整个研究机构自1998年以来一直没有病毒抗体，自1998年以来，严重的免疫缺陷小鼠被繁殖，并在FCCC研究中成功生存了两年。肺炎藻，巴斯德氏菌和螺旋杆菌已经从所有育种和免疫缺陷型啮齿动物区域中消除，并且持续的系统重新培养以及显微溶解剂增加的外壳有望在两年内完全从所有FCCC菌落中完全消除这些药物。 2003年，LAHF进行了11,967次测试（5,600个微生物培养物，3,179种ELISA测定法，2,088（PCR）测定法和1,100个尸检，直接支援研究人员的直接服务时间数量增加了210％（2003年）。从1998年（616个服务时间）到2003年，对动物的维持，肿瘤维持，肿瘤和细胞培养筛查和微生物学/血清学测试的需求将近四倍（1,912个服务时间）。