CORE--RESEARCH CYTOGENETICS

核心--研究细胞遗传学

• 批准号: 7142523
• 负责人: Robert C Young
• 金额: $ 8.18万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-10 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7142523
• 关键词: biomedical facility; cytogenetics; karyotype; laser capture microdissection; molecular oncology

The Research Cytogenetics and Laser Capture Microdissection (RCLC) Facility provides karyolyping, molecular cytogenetic analyses, and Laser Capture Microdissection (LCM). The RCLC Facility, established in July 1995, is used by Members of all three FCCC Divisions from 11 research Programs. The Facility supported 29 funded investigators over the last five years. In addition to offering a full spectrum of cytogenetic services, the RCLC Facility has added multiplex-fluorescence In situ hybridization FISH (M-FISH) for analysis of structural rearrangements and array-comparative genomic hybridization (array-CGH) for analysis of DNA copy number changes in tumor specimens and cell lines. Two complete LCM workstations are now available, including a new AutoPix LCM apparatus (Arcturus) that provides rapid and convenient access to pure cell populations through a totally automated system. The LCM component is managed by a trained pathologist. The Facility Director is the Program Leader of Human Genetics and the cytogenetics component is managed by an experienced and skilled cytogeneticist. In 2003, the number of tests performed by the Research Cytogenetics component increased nearly four-fold compared to that carried out in 1999, when this was a Developing Core Facility. In 1999, we performed conventional FISH mapping and karyotypic and CGH analyses on 20 samples, whereas in 2003, 73 samples were analyzed, an increase of 265%. From the time when LCM services were first introduced in the RCLC Facility four years ago, LCM usage has increased from 150 hours in 2000 to 570 hours in 2003, an increase of 280%. The percentage of the Facility's operating budget supported by user's fees increased from 11% in 1999 to 20% in 2003. Although demand for FISH mapping of native gene loci has virtually ended, requests increased by 160% for FISH analysis of structural rearrangements or viral/transgene integration sites, interphase FISH to identify genomic alterations in non-dividing cells, and chromosome painting and M-FISH to facilitate interpretation of complex or subtle chromosome rearrangements. In addition, requests for karyotyping of embryonic stem cell clones used in mouse model studies is a new frequent demand averaging 24 requests per year.

研究细胞遗传学和激光捕获显微解剖（RCLC）设施提供 核分型，分子细胞遗传学分析和激光捕获显微解剖（LCM）。这 RCLC设施成立于1995年7月，由11个FCCC部门的成员使用。 研究计划。该设施在过去五年中支持了29名资助的调查人员。在 除了提供各种细胞遗传学服务外，RCLC设施还增加了多重荧光原位杂交鱼（M-FISH），以分析结构重排和阵列比较基因组杂交（ARRAY-CGH），以分析肿瘤标本和细胞线中DNA拷贝数的分析。现在可以使用两个完整的LCM工作站，包括新的Autopix LCM设备（Arcturus），该设备可通过完全自动化的系统快速快速访问纯细胞种群。 LCM组件由训练有素的病理学家管理。该设施主管是人类遗传学的计划负责人，细胞遗传学成分由经验丰富且熟练的细胞遗传学家管理。在2003年，研究细胞遗传学成分进行的测试数量与1999年进行的核心设施相比增加了近四倍。 1999年，我们对20个样本进行了常规的鱼类图和核型和CGH分析，而在2003年，分析了73个样本，增加了265％。从四年前首次在RCLC设施中引入LCM服务的时间，LCM使用率已从2000年的150小时增加到2003年的570小时，增加了280％。用户费用支持的设施的运营预算百分比从1999年的11％增加到2003年的20％。尽管对本地基因基因座的鱼类映射的需求实际上已经结束，但请求增加了160％，用于鱼类的结构重排或病毒/转基因整合位点的结构重排或偶相互为绘画细胞和cr绘画的植物性变化，并鉴定出偶然的细胞和chromose primation and-fister和mosos绘画，并识别涂漆的构成和杂物。染色体重排。此外，在小鼠模型研究中使用的胚胎干细胞克隆的核分型请求是每年平均24个请求的新需求。