CORE--CYTOGENETICS

核心--细胞遗传学

• 批准号: 6989951
• 负责人: ROBERT B. JENKINS
• 金额: $ 8.39万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6989951
• 关键词: biomedical facility; comparative genomic hybridization; cytogenetics; fluorescence microscopy; fluorescent in situ hybridization; karyotype; neoplasm /cancer; neoplasm /cancer genetics; oncology

With the recognition that cancer is a genetic disease, many cancer researchers, including scientists in the Mayo Clinic Cancer Center (MCCC), require access to routine and advanced cytogenetics services. These services include routine cytogenetic analysis, spectral karyotype (SKY) analyses of metaphases, interphase and metaphase fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH), and schedulable fluorescent microscope and imaging system time. Such services can aid in the identification of genetic regions important for the development of specific malignancies. Such services can also be used to characterize the clonality of tumor specimens and determine the origin (e.g., mouse vs. human) of a specific tumor culture. The shared resource has supported the research of over 30 Cancer Center members during the past 5-year funding period, performing routine cytogenetic, CGH, SKY and FISH analyses on over 100,70, 30 and 900 cases, respectively. The shared resource plans to add comparative genomic hybridization to array (CGHa) services. Thus, the Cytogenetics Shared Resource provides routine cytogenetic and advanced molecular cytogenetic services to MCCC members.

认识到癌症是一种遗传疾病，许多癌症研究人员，包括梅奥临床癌症中心（MCCC）的科学家，都需要获得常规和晚期细胞遗传学服务。这些服务包括常规的细胞遗传学分析，中期的光谱核型（SKY）分析，相间和中期荧光原位杂交（FISH），比较基因组杂交（CGH）以及可计划的荧光显微镜和成像系统时间。这样的服务可以帮助识别对特定恶性肿瘤发展重要的遗传区域。此类服务也可以用来表征肿瘤标本的克隆性，并确定特定的特定的起源（例如，小鼠与人） 肿瘤培养。共享资源在过去的5年资金期间支持了30多个癌症中心成员的研究，分别对超过100,70、30和900例进行了常规的细胞遗传学，CGH，天空和鱼类分析。共享资源计划将比较基因组杂交添加到阵列（CGHA）服务。因此，细胞遗传学共享资源为MCCC成员提供了常规的细胞遗传学和晚期分子细胞遗传学服务。