Cytogenetics

细胞遗传学

基本信息

批准号：
7944991
负责人：
ROBERT B. JENKINS
金额：
$ 9.99万
依托单位：
MAYO CLINIC ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-17 至 2014-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7944991
关键词：
Animal Model Applications Grants Cancer Center Cancer Center Support Grant Cell Culture Techniques Cells Chromosome Breakage Clinical Clonality Color Comprehensive Cancer Center Cultured Cells Custom Cytogenetic Analysis Cytogenetics Cytogenetics Shared Resource Data Analyses Development Diagnostic Disease Embryo Fibroblasts Fluorescent in Situ Hybridization Freezing Genes Genetic Grant Hereditary Disease Home environment Human Hybrid Cells Image Interphase Laboratories Location Malignant Neoplasms Maps Mayo Clinic Cancer Center Metaphase Microscope Molecular Cytogenetics Monitor Mus Paraffin Embedding Pattern Process Production Prognostic Marker Publications Research Research Personnel Resource Sharing Sampling Scientist Services Slide Specimen Spectral Karyotyping System Techniques Tissue Microarray Tissues Transgenes Tumor Cell Line Visual Aid Xenograft procedure cell type established cell line fluorescence microscope meetings member nerve stem cell novel programs research study tumor

项目摘要

The central focus of the Cytogenetics Shared Resource (CSR) is to provide cytogenetic and molecular cytogenetic analysis of human and animal model research samples. These samples may be in the form of cultured cells, fresh or frozen tissue, paraffin-embedded sections, or tissue microarrays (TMAs). Specific cell types include tumor cell lines, xenograft cells, hybrid cells from chimeric animal models, neural stem cells, and mouse embryonic fibroblasts, among others. The CSR provides specialized services including cell culture, routine cytogenetic analysis, chromosome breakage analysis, interphase and metaphase Fluorescence in situ Hybridization (FISH), Spectral Karyotyping (SKY) analysis of human and mouse metaphases, and homebrew FISH Probe Production. This last service provides novel FISH probes that are specific to investigators needs and are usually not commercially available. These probes are then used for FISH analysis studies. The CSR is expanding this service beyond homebrew enumeration FISH probes by creating custom translocation/fusion probes, break-apart probes, and multi-color FISH probes to ultimately assist in developing strategic probes that become clinical diagnostic and prognostic markers of disease. Other services provided by the CSR to meet the needs of investigators include utilization of the CSR laboratory for slide processing and fluorescent microscope and imaging system use. Briefly, applications of CSR services include: to characterize the clonality of tumor specimens; to determine the origin of a specific tumor culture (i.e., mouse vs. human), to aid in the identification of genetic regions important for the development of specific malignancies, and to map the location of DMA sequences, genes, or transgene insertions. With the recognition that cancer is a genetic disease, many cancer researchers, including scientists in the Mayo Clinic Cancer Center (MCCC), require access to CSR services. This Shared Resource is utilized by 9 of the 12 Cancer Center Programs. Approximately 66% of CSR users are CCSG members and represent 92% of the overall utilization.

细胞遗传学共享资源 (CSR) 的中心重点是提供细胞遗传学和分子生物学人类和动物模型研究样本的细胞遗传学分析。这些样本可以是以下形式培养细胞、新鲜或冷冻组织、石蜡包埋切片或组织微阵列 (TMA)。特定细胞类型包括肿瘤细胞系、异种移植细胞、来自嵌合动物模型的杂交细胞、神经干细胞、和小鼠胚胎成纤维细胞等。 CSR提供专业服务，包括细胞培养、常规细胞遗传学分析、染色体断裂分析、间期和中期人类和小鼠的荧光原位杂交 (FISH)、光谱核型分析 (SKY) 中期和自制 FISH 探针生产。最后一项服务提供新颖的 FISH 探针专门针对研究人员的需求，通常无法在商业上获得。然后这些探针用于 FISH 分析研究。 CSR 正在将这项服务扩展到自制枚举 FISH 探针之外：创建定制易位/融合探针、分离探针和多色 FISH 探针，最终协助开发成为疾病临床诊断和预后标志物的战略探针。 CSR 为满足调查人员的需求而提供的其他服务包括利用 CSR 实验室供载玻片处理以及荧光显微镜和成像系统使用。简而言之，应用 CSR 服务包括：表征肿瘤样本的克隆性；以确定特定的来源肿瘤培养（即小鼠与人类），以帮助识别对肿瘤重要的遗传区域特定恶性肿瘤的发展，并绘制 DMA 序列、基因或转基因的位置图插入。随着癌症是一种遗传性疾病的认识，许多癌症研究人员，包括梅奥诊所癌症中心 (MCCC) 的科学家需要获得企业社会责任服务。此共享资源 12 个癌症中心项目中的 9 个使用该项目。大约 66% 的 CSR 用户是 CCSG 成员占总体利用率的 92%。