CORE--HISTOPATHOLOGY

核心--组织病理学

• 批准号: 7142512
• 负责人: Robert C Young
• 金额: $ 7.61万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-10 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7142512
• 关键词: biomedical facility; histopathology; immunocytochemistry; in situ hybridization; neoplasm /cancer; tissue /cell preparation

The purpose of the Histopathology Facility (HF) is to facilitate research conducted by investigators with peer-reviewed funding at FCCC by providing them with technical processing of cells and tissues as well as helping in the interpretation of results. The Facility's Technicians and Pathologist play a major support role in numerous research projects at the Center and their contributions have been and continue to be of paramount importance in the establishment and characterization of human cancer and other disease models. The Facility prepares, processes, and assists in evaluating tissues derived from experimental protocols developed by investigators with peer-reviewed funding. Most of the projects involve animal models of human cancer that require complex histological and/or cytological processing to determine morphological alterations, as well as to localize cancer-relevant gene products. The most frequently utilized services of the Facility include: laboratory animal autopsy, fixation, embedding and sectioning of paraffin-embedded tissues (7,000-8,000 per year), unstained paraffin sections for immunohistocnemistry (IHC) and LCM (10,000-14,000 per year), cryomicrotomy (100-200 per year), special stains (500-700 per year), immunohistochemistry (2,500-3,500 per year), in situ hybridization (100-200 per year), autoradiography (100-200 per year), digital microphotography (1,000-2,500 per year), and interpretative histopathology (7,000-10,000 HE and IHC slides signed-out per year). During the last CCSG funding cycle we have seen a significant increase in all services rendered as exemplified by the doubling in number of embedded and sectioned tissues (110% increase) and in the immunohistochemical work (120% increase). Furthermore, during 2000-2004 we implemented new technology such as tissue microarray sectioning and a Web-based Experimental Histopathology database. The Histopathology Facility also provides support to the Laboratory Animal Health Facility in quality control and animal health monitoring activities, as well as processing support to the Tumor Bank and the Research Cytogenetics and Laser Capture Microdissection Facilities. The HF is used by 33 investigators with peer-reviewed funding in 10 different Programs from all three Divisions. Eighty-nine percent (86%) of its use is for studies supported with peer-reviewed funding.

组织病理学设施（HF）的目的是促进由 通过为FCCC进行同行评审的资金的研究人员通过为细胞和组织的技术处理以及帮助解释结果来进行资金。该设施的技术人员和病理学家在该中心的众多研究项目中发挥了重要的支持作用 在机构中，捐款一直至关重要 人类癌症和其他疾病模型的表征。该设施准备，过程和有助于评估来自由研究人员开发的实验方案的组织，并经过同行评审的资金。大多数项目涉及需要复杂的组织学和/或细胞学加工来确定形态学改变以及定位与癌症相关的基因产物的动物模型。该设施的最常使用的服务包括：实验室动物尸检，固定，嵌入和切片 - 石蜡包裹的组织（每年7,000-8,000），未染色的免疫组织循环切片（IHC）和LCM（每年10,000-14,000）（每年10,000-14,000），每年10,000-14,000，年度cryomicicrotomy（100-200）（100-200）（100-200）（100-200），（100-200），state（100-200），state（100-200），（state），state（100-200），stat（state）免疫组织化学（每年2,500-3,500），原位杂交（每年100-200次），放射自显影（每年100-200次），数字显微光检查（每年1,000-2,500）和解释性组织病理学（7,000-10,000 HE和IHC签名每年签名）。在上一个CCSG融资周期中，我们看到的所有服务都显着增加，这是嵌入式组织和切片组织数量增加一倍（增加110％）以及免疫组织化学工作（增长120％）。此外，在2000 - 2004年期间，我们实施了新技术，例如组织微阵列切片和基于Web的实验组织病理学数据库。组织病理学设施还为实验室动物健康机构提供了质量控制和动物健康监测活动的支持，以及对肿瘤库以及研究细胞遗传学和激光捕获微分解剖设施的加工支持。 33名调查员使用HF在所有三个部门的10个不同计划中进行了同行评审的资金。其使用量为89％（86％）用于接受同行评审的资金支持的研究。