CORE--CELL CULTURE

核心--细胞培养

• 批准号: 7142502
• 负责人: Robert C Young
• 金额: $ 16.18万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-10 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7142502
• 关键词: biomedical facility; embryo /fetus tissue /cell culture; neoplasm /cancer; tissue /cell culture

Cell culture technology plays an essential role in modern biomedical research, especially in cancer research. The Cell Culture Facility (CCF) serves as a customized pay-for-service Facility that provides expertise, technical support, reagents, equipment, liquid nitrogen storage, and/or training to increase the effectiveness and efficiency of using mammalian cell culture in FCCC laboratories. This Facility provides technical assistance and consultation services in tissue culture techniques, centralized liquid nitrogen banking, mycoplasma screening services, the preparation of custom and standard culture media and supplements, performance testing of fetal bovine serum, cell propagation, and technical support and supplies for mouse embryonic stem (ES) cell culture. Major efforts by the CCF include carrying out technically-challenging techniques, such as the establishment of primary cell cultures from patient or animal tissue samples and the generation of gene "knockout" or "knockin" ES cells. In 2003, the Facility served 52 investigators with peer-reviewed funding and a total of 56 of the 65 research laboratories at FCCC from all three Divisions and 11 Research Programs. Peer-reviewed, funded research accounted for more than 92.5% of CCF usage. The Facility is highly-regarded among faculty users, and received an "Outstanding to Excellent" rating at the last CCSG review. In the past five years, the demand for CCF services has increased substantially, with 225% more primary cell lines established and 54-117% increases in the distribution of media, sterile reagents, and serum. Importantly, the Facility supplies the FCCC community with valuable on-site expertise for a host of established cell culture protocols, consultation in the design and evaluation of experiments, training in cell culture methodologies, and adaptable assistance delivered in a cost-effective manner. The Facility continues to provide innovative services, including the recent establishment of "nucleofection" services to efficiently transfect cell lines and the production of extracellular matrices for "three-dimensional" cell culture. Thus, the CCF serves FCCC investigators with a ready source of expertise, manpower, and a convenient and cost-effective resource for culture supplies and specialized services.

细胞培养技术在现代生物医学研究中发挥着至关重要的作用，特别是在 癌症研究。细胞培养设施 (CCF) 作为定制的付费服务设施 提供专业知识、技术支持、试剂、设备、液氮储存和/或 提高在 FCCC 中使用哺乳动物细胞培养的有效性和效率的培训 实验室。该设施提供组织培养技术、集中液氮库、支原体筛查服务、定制和标准培养基和补充剂的制备、胎牛血清性能测试、细胞增殖以及小鼠技术支持和用品等方面的技术援助和咨询服务。胚胎干（ES）细胞培养。 CCF 的主要工作包括实施技术上具有挑战性的技术，例如从患者或动物组织样本中建立原代细胞培养物以及生成基因“敲除”或“敲入”ES 细胞。 2003 年，该设施为 52 名研究人员提供了同行评审资金，并为 FCCC 65 个研究实验室中来自所有三个部门和 11 个研究项目的总共 56 个实验室提供服务。经同行评审、资助的研究占 CCF 使用量的 92.5% 以上。该设施受到教师用户的高度评价，并在上次 CCSG 审查中获得了“杰出到优秀”的评级。在过去五年中，对 CCF 服务的需求大幅增加，原代细胞系的建立增加了 225%，培养基、无菌试剂和血清的分配增加了 54-117%。重要的是，该设施为 FCCC 社区提供了宝贵的现场专业知识，涉及一系列已建立的细胞培养方案、实验设计和评估方面的咨询、细胞培养方法的培训以及以具有成本效益的方式提供的适应性援助。该设施继续提供创新服务，包括最近建立的“核转染”服务，以有效转染细胞系以及生产用于“三维”细胞培养的细胞外基质。因此，CCF 为 FCCC 调查人员提供现成的专业知识、人力以及方便且具有成本效益的文化供应和专业服务资源。