CORE--CELL CULTURE

核心--细胞培养

• 批准号: 7142502
• 负责人: Robert C Young
• 金额: $ 16.18万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-10 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7142502
• 关键词: biomedical facility; embryo /fetus tissue /cell culture; neoplasm /cancer; tissue /cell culture; biomedical facility; embryo /fetus tissue /cell culture; neoplasm /cancer; tissue /cell culture

Cell culture technology plays an essential role in modern biomedical research, especially in cancer research. The Cell Culture Facility (CCF) serves as a customized pay-for-service Facility that provides expertise, technical support, reagents, equipment, liquid nitrogen storage, and/or training to increase the effectiveness and efficiency of using mammalian cell culture in FCCC laboratories. This Facility provides technical assistance and consultation services in tissue culture techniques, centralized liquid nitrogen banking, mycoplasma screening services, the preparation of custom and standard culture media and supplements, performance testing of fetal bovine serum, cell propagation, and technical support and supplies for mouse embryonic stem (ES) cell culture. Major efforts by the CCF include carrying out technically-challenging techniques, such as the establishment of primary cell cultures from patient or animal tissue samples and the generation of gene "knockout" or "knockin" ES cells. In 2003, the Facility served 52 investigators with peer-reviewed funding and a total of 56 of the 65 research laboratories at FCCC from all three Divisions and 11 Research Programs. Peer-reviewed, funded research accounted for more than 92.5% of CCF usage. The Facility is highly-regarded among faculty users, and received an "Outstanding to Excellent" rating at the last CCSG review. In the past five years, the demand for CCF services has increased substantially, with 225% more primary cell lines established and 54-117% increases in the distribution of media, sterile reagents, and serum. Importantly, the Facility supplies the FCCC community with valuable on-site expertise for a host of established cell culture protocols, consultation in the design and evaluation of experiments, training in cell culture methodologies, and adaptable assistance delivered in a cost-effective manner. The Facility continues to provide innovative services, including the recent establishment of "nucleofection" services to efficiently transfect cell lines and the production of extracellular matrices for "three-dimensional" cell culture. Thus, the CCF serves FCCC investigators with a ready source of expertise, manpower, and a convenient and cost-effective resource for culture supplies and specialized services.

细胞培养技术在现代生物医学研究中起着至关重要的作用，尤其是 癌症研究。细胞培养设施（CCF）用作定制的薪酬服务设施 提供专业知识，技术支持，试剂，设备，液氮存储和/或 训练以提高使用哺乳动物细胞培养在FCCC中的有效性和效率 实验室。该设施在组织培养技术，集中液氮库，支原体筛查服务，定制和标准培养基的准备，胎牛血清的性能测试，细胞传播以及针对小鼠胚胎的技术支持和供应小鼠胚胎（ES）细胞培养物中提供技术援助和咨询服务。 CCF的重大努力包括进行技术挑战的技术，例如从患者或动物组织样本中建立原代细胞培养物以及产生基因“基因敲除”或“敲除” ES细胞。 2003年，该设施为52名调查人员提供了同行评审的资金，在FCCC的65个研究实验室中，总共有56名来自所有三个部门和11个研究计划。经过同行评审的资助研究占CCF使用情况的92.5％以上。该设施在教职用户中受到高度尊敬，并在上次CCSG评论中获得了“出色的优秀”评级。在过去的五年中，对CCF服务的需求已大大增加，建立了225％的主要细胞系，培养基，无菌试剂和血清的分布增加了54-117％。重要的是，该设施为FCCC社区提供了有价值的现场专业知识，为许多既定的细胞培养方案，对实验的设计和评估进行咨询，细胞培养方法的培训以及以具有成本效益的方式提供的适应性援助。该设施继续提供创新的服务，包括最近建立“核反理”服务以有效转染细胞系以及用于“三维”细胞培养的细胞外矩阵。因此，CCF为FCCC调查人员提供了现成的专业知识，人力的来源，以及方便且具有成本效益的文化供应和专业服务资源。