Genome Sequencing

基因组测序

基本信息

批准号：
10245046
负责人：
Erik A Lundquist
金额：
$ 21.02万
依托单位：
UNIVERSITY OF KANSAS LAWRENCE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-07-15 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10245046
关键词：
Affect Animal Model Area Bioinformatics Biological Sciences Biology Biomedical Engineering Biomedical Research Businesses Cell physiology Centers of Research Excellence Chromatin Communication Core Facility Custom DNA Sequencing Facility DNA sequencing Data Development Discipline Disease Disease Pathway Dissection Ecology Elements Experimental Designs Fluorescence Fluorescent Probes Food Generations Genes Genetic Genome Genomics Genotype Goals Infrastructure Institution Iowa Kansas Libraries Location Methods Microarray Analysis Molecular Molecular Analysis Museums Mutation Natural History Network Infrastructure Partner in relationship Pathway interactions Pharmacy Schools Preparation Protocols documentation Research Research Personnel Running Sequence Analysis Signal Pathway Site Small RNA Synthesis Chemistry Techniques Technology Training Universities Variant Washington Wichita Work chromatin immunoprecipitation cost experimental analysis genome sequencing higher education instrument interest next generation sequencing novel novel strategies outreach tool transcription factor transcriptome transcriptome sequencing whole genome

Affect Animal Model Area Bioinformatics Biological Sciences Biology Biomedical Engineering Biomedical Research Businesses Cell physiology Centers of Research Excellence Chromatin Communication Core Facility Custom DNA Sequencing Facility DNA sequencing Data Development Discipline Disease Disease Pathway Dissection Ecology Elements Experimental Designs Fluorescence Fluorescent Probes Food Generations Genes Genetic Genome Genomics Genotype Goals Infrastructure Institution Iowa Kansas Libraries Location Methods Microarray Analysis Molecular Molecular Analysis Museums Mutation Natural History Network Infrastructure Partner in relationship Pathway interactions Pharmacy Schools Preparation Protocols documentation Research Research Personnel Running Sequence Analysis Signal Pathway Site Small RNA Synthesis Chemistry Techniques Technology Training Universities Variant Washington Wichita Work chromatin immunoprecipitation cost experimental analysis genome sequencing higher education instrument interest next generation sequencing novel novel strategies outreach tool transcription factor transcriptome transcriptome sequencing whole genome

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项目摘要

PROJECT SUMMARY Next generation sequencing is now a central tool in the analysis of signaling pathways important in development and disease. The Genome Sequencing Core (GSC) is aimed at providing researchers at the University of Kansas and the state of Kansas with next-generation sequencing technologies as well as expertise in experimental design and analysis of sequence data. Projects in the GSC include whole genome assembly, genome re-sequencing for identification of mutations important in development and disease, transcriptome analysis (RNA seq), variant mapping and genotyping, and identification of transcription factor interaction sites using chromatin immunoprecipitation combined with DNA sequencing (ChIP seq). The GSC houses an Illumina Hiseq 2500 sequencer and a MiSeq sequencer with dedicated processor and storage space to run the instrument. This core facility has enhanced the genomics infrastructure already at KU, in the KU Genomics Center and the KU DNA Sequencing Facility, which provides standard, first generation sequencing. Together, the three cores allow for a thorough, integrated dissection of disease pathways using novel fluorescence probes to define a cellular process, to find mutations affecting that cellular process, and to define genes involved in the cellular process. The novel approach of the CMADP is to combine the enabling technologies of fluorescent probe synthetic chemistry and next generation sequencing to set up a pipeline for target discovery in disease pathways. Next generation sequencing is an enabling technology in that it has the power to allow investigators from many different disciplines to ask new questions in their research areas of interest. The GSC enables genomics research at the University of Kansas by eliminating the barriers of cost and communication involved with using off-campus facilities. Interest in next generation sequencing at the University of Kansas is high, and includes investigators in the Pharmacy School, the Department of Molecular Biosciences, and the Department of Ecology and Evolutionary Biology. Use of the GSC will be by Projects in the COBRE Center for Molecular Analysis of Disease Pathways, as well as by researchers across the State of Kansas and in many different disciplines.

项目摘要现在，下一代测序是分析信号通路的中心工具发展与疾病。基因组测序核心（GSC）旨在为研究人员提供堪萨斯大学和堪萨斯州采用下一代测序技术以及实验设计和序列数据分析方面的专业知识。 GSC中的项目包括整个基因组组装，重新测序以鉴定在发育和疾病中重要的突变，转录组分析（RNA SEQ），变体映射和基因分型以及转录因子的鉴定使用染色质免疫沉淀与DNA测序（CHIP SEQ）结合的相互作用位点。 GSC 设有一个Illumina Hiseq 2500音序器和带有专用处理器和存储的Miseq Sequencer 运行乐器的空间。该核心设施增强了KU，KU基因组中心和 KU DNA测序设施提供了标准的第一代测序。在一起，三个核心允许使用新型的荧光探针对疾病途径进行彻底的，整合的解剖定义一个细胞过程，以找到影响细胞过程的突变，并定义涉及的基因细胞过程。 CMADP的新方法是结合荧光的能力技术探针合成化学和下一代测序，以建立疾病目标发现的管道途径。下一代测序是一种使技术，因为它有能力允许调查人员许多不同的学科在他们感兴趣的研究领域提出新问题。 GSC启用基因组学通过消除使用的成本和沟通障碍，在堪萨斯大学的研究校外设施。堪萨斯大学对下一代测序的兴趣很高，其中包括药房，分子生物科学系和部门的研究人员生态和进化生物学。 GSC的使用将是通过鞋底分子中心的项目进行的疾病途径以及堪萨斯州和许多不同不同的研究人员分析学科。