CORE--ZEBRAFISH GENETICS AND AQUARIUM

核心--斑马鱼遗传学和水族馆

• 批准号: 6613924
• 负责人: JOHN H. POSTLETHWAIT
• 金额: $ 27.67万
• 依托单位: UNIVERSITY OF OREGON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-18 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6613924
• 关键词: alternatives to animals in research; animal breeding; animal care; animal colony; biological signal transduction; biomedical facility; biotechnology; developmental genetics; egg /ovum; embryo /fetus; gene mutation; genetic manipulation; genetic registry /resource /referral center; genetic screening; mutant; radiation genetics; vertebrate embryology; zebrafish

Genetics Facility: The primary objectives of the Genetics Facility are: 1) ENU mutagenesis; 2) providing mutagenized stocks for screens described separately in each of our Component Projects, and 3) providing pathenogenetic offspring, both androgenetic and gynogenetic, for mutation screens. Aquarium Facility: The objectives of the zebrafish Aquarium Facility are to provide: 1) husbandry of fish stocks; 2) quarantine facilities for newly acquired stocks; 3) staged eggs; 4) procedure rooms and equipment for microinjection of eggs; 5) a liquid nitrogen (LN) freezer for storing frozen sperm; 6) databases on the facility's fish stock collections; and 7) advice and training on fish husbandry and facility set-up.

遗传学设施：遗传学设施的主要目标是： 1) ENU 诱变； 2) 为我们每个组成项目中单独描述的筛选提供诱变的种群，以及 3) 为突变筛选提供雄性遗传和雌核遗传的致病后代。水族馆设施：斑马鱼水族馆设施的目标是提供： 1) 鱼类种群的饲养； 2）新进库存检疫设施； 3）分阶段彩蛋； 4）卵子显微注射手术室和设备； 5)液氮(LN)冷冻机，用于储存冷冻精子； 6) 设施鱼类资源收集数据库； 7) 有关鱼类养殖和设施设置的建议和培训。