Core--Mouse Genetics

核心--小鼠遗传学

• 批准号: 6946745
• 负责人: Catherine C Hedrick
• 金额: $ 14.2万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-16 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6946745
• 关键词: alternatives to animals in research; animal breeding; animal care; animal colony; biomedical facility; bone marrow transplantation; genetically modified animals; genotype; inflammation; laboratory mouse; lung injury

A primary function of the Mouse Core is to breed, maintain and genotype mice used within the PPG. Dr. Hedrick has extensive experience in the breeding of transgenic and knockout animals and in the maintenance of mouse colonies. The Mouse Core will maintain and genotype all mouse colonies that are used by more than one project. The Core will be responsible for all purchase of commercially available mice needed (such as study controls) for all projects. A secondary function of the Core is to perform bone marrow transplantations in mice. In this case, marrow will be extracted by the Core, and injected into irradiated recipients. The irradiation will be performed by each Project; however after irradiation, marrow injections, mouse housing, and mouse care will be performed by Core personnel. Once the mice have recovered from irradiation, the mice will be provided to each Project for experimentation. In order to keep the numbers and use of mice as limited as is possible, there will be considerable sharing of mouse tissues among the four projects. For example, mice used for obtaining lung endothelial cells can also be used to obtain splenocytes. Other tissues from the mice will be shared among the Projects as needed for immunostaining and microarray analysis.

Mouse Core 的主要功能是对 PPG 中使用的小鼠进行繁殖、维持和基因分型。 Hedrick 博士在转基因和基因敲除动物的培育以及小鼠群体的维护方面拥有丰富的经验。 Mouse Core 将维护多个项目使用的所有小鼠群体并对其进行基因分型。核心将负责所有项目所需的所有市售小鼠（例如研究对照）的采购。 Core 的第二个功能是对小鼠进行骨髓移植。在这种情况下，骨髓将被核心提取，并注射到受辐射的接受者体内。照射将由每个项目进行；然而，照射后，骨髓注射、小鼠饲养和小鼠护理将由核心人员进行。一旦老鼠恢复过来 照射后，将小鼠提供给各项目进行实验。为了尽可能限制小鼠的数量和使用，这四个项目将共享大量的小鼠组织。例如，用于获得肺内皮细胞的小鼠也可以用于获得脾细胞。小鼠的其他组织将根据免疫染色和微阵列分析的需要在项目之间共享。