Metabolic Studies of Mouse Models of Obesity & Diabetes

肥胖小鼠模型的代谢研究

• 批准号: 7153591
• 负责人: Oksana Gavrilova
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7153591
• 关键词: NOD mouse; body composition; body temperature; carbohydrate metabolism; diabetes mellitus; disease /disorder model; fatty acid metabolism; fatty acids; glucagon; glucose clamp technique; glucose tolerance; glutamates; hormone metabolism; human tissue; insulin; insulin sensitivity /resistance; metabolism; nutrient intake activity; obesity; pancreatic islet function; pancreatic islet transplantation; pyruvates; thermogenesis

Diabetes and obesity are very common in Western society. In spite of their prevalence, our understanding of their molecular basis is rudimentary. Mouse models of obesity and diabetes contribute greatly to a better understanding of how the environmental and genetic causes affect metabolism. Mouse Metabolic Core has developed a set of tools to analyze metabolism in genetically modified mice. The following techniques are now provided as service to the NIDDK investigators. To characterize obesity we analyze body composition in live mice, body temperature, food intake, metabolic rates and diet induced thermogenesis. To study diabetes we perform insulin tolerance, glucose tolerance, 1st phase insulin secretion tests, glucagon, pyruvate and glutamate challenge tests, and conduct euglycemic-hyperinsulinemic clamp. To characterize pancreatic islets function we isolate islets from mouse pancreas, induce type 1 diabetes using STZ and perform transplantation of islets from various sources into NOD scid diabetic mice. We also measure the rates of triglyceride clearance and production, analyze tissue triglyceride content and fatty acid oxidation in isolated muscle, perform hormone clearance tests and measure concentration of major metabolites and hormones in mouse serum.

糖尿病和肥胖症在西方社会非常普遍。尽管它们很普遍，但我们对其分子基础的了解还很初级。肥胖和糖尿病的小鼠模型极大地有助于更好地了解环境和遗传原因如何影响新陈代谢。 Mouse Metabolic Core 开发了一套工具来分析转基因小鼠的新陈代谢。现在向 NIDDK 调查人员提供以下技术作为服务。为了表征肥胖，我们分析了活体小鼠的身体成分、体温、食物摄入量、代谢率和饮食诱导的生热作用。为了研究糖尿病，我们进行胰岛素耐量、葡萄糖耐量、第一阶段胰岛素分泌测试、胰高血糖素、丙酮酸和谷氨酸激发测试，并进行正常血糖-高胰岛素钳夹。为了表征胰岛功能，我们从小鼠胰腺中分离胰岛，使用 STZ 诱导 1 型糖尿病，并将各种来源的胰岛移植到 NOD scid 糖尿病小鼠中。我们还测量甘油三酯的清除率和产生率，分析离体肌肉中的组织甘油三酯含量和脂肪酸氧化，进行激素清除测试并测量小鼠血清中主要代谢物和激素的浓度。