Kansas Center for Metabolism and Obesity REsearch (KC-MORE) - Metabolism Core

堪萨斯代谢与肥胖研究中心 (KC-MORE) - 代谢核心

基本信息

批准号：
10598029
负责人：
Paige C Geiger
金额：
$ 29.71万
依托单位：
UNIVERSITY OF KANSAS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-04-01 至 2027-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10598029
关键词：
Adipocytes Adipose tissue Behavior Behavioral Bioenergetics Biological Models Body Composition Body measure procedure Carbohydrates Cell Size Cells Cellular Metabolic Process Chronic Chronic Disease Core Facility Coupling Diet Dietary intake Disease Energy Intake Energy Metabolism Environmental Risk Factor Equipment Exercise Fasting Fatty Acids Fatty acid glycerol esters Food Intake Regulation Genus Hippocampus Glucose Homeostasis Human Impairment Individual Insulin Resistance Isotope Labeling Isotopes Kansas Liver Macronutrients Nutrition Measurement Measures Mediating Metabolic Metabolic Diseases Metabolic dysfunction Metabolism Methodology Mission Mitochondria Mus Muscle Obesity Obesity associated disease Outcome Measure Oxygen Consumption Palmitates Pancreas Participant Pathologic Physical activity Play Pre-Clinical Model Predisposition Rattus Reactive Oxygen Species Research Research Personnel Resources Rodent Rodent Model Role Sampling Scientist Services Sleep System Temperature Testing Thermogenesis Time Time-restricted feeding Tissues Tracer Treatment Protocols Weight Gain design diet and exercise dietary manipulation energy balance human model in vivo insulin tolerance metabolic abnormality assessment metabolic phenotype new therapeutic target obesity development oxidation pre-clinical tool translational study

Adipocytes Adipose tissue Behavior Behavioral Bioenergetics Biological Models Body Composition Body measure procedure Carbohydrates Cell Size Cells Cellular Metabolic Process Chronic Chronic Disease Core Facility Coupling Diet Dietary intake Disease Energy Intake Energy Metabolism Environmental Risk Factor Equipment Exercise Fasting Fatty Acids Fatty acid glycerol esters Food Intake Regulation Genus Hippocampus Glucose Homeostasis Human Impairment Individual Insulin Resistance Isotope Labeling Isotopes Kansas Liver Macronutrients Nutrition Measurement Measures Mediating Metabolic Metabolic Diseases Metabolic dysfunction Metabolism Methodology Mission Mitochondria Mus Muscle Obesity Obesity associated disease Outcome Measure Oxygen Consumption Palmitates Pancreas Participant Pathologic Physical activity Play Pre-Clinical Model Predisposition Rattus Reactive Oxygen Species Research Research Personnel Resources Rodent Rodent Model Role Sampling Scientist Services Sleep System Temperature Testing Thermogenesis Time Time-restricted feeding Tissues Tracer Treatment Protocols Weight Gain design diet and exercise dietary manipulation energy balance human model in vivo insulin tolerance metabolic abnormality assessment metabolic phenotype new therapeutic target obesity development oxidation pre-clinical tool translational study

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项目摘要

METABOLISM CORE: PROJECT SUMMARY Metabolism plays a fundamental role in the development of obesity and the pathological consequences of obesity that lead to chronic disease. Dysregulation of energy balance and impaired storage or oxidation of substrates are fundamental features of developing obesity and metabolic disease. Metabolic dysregulation is driven by both alterations in cellular mitochondrial function and disruptions in whole body metabolic homeostasis. Therefore, metabolic research that connects cellular mitochondrial energetics to whole body in vivo substrate metabolism and energy expenditure is critical for understanding mechanisms underlying metabolic dysfunction, obesity, and obesity-related disease states. The Metabolism (MET) core will provide the Kansas Center for Metabolism and Obesity REsearch (KC-MORE) with expertise, methodologies, and equipment for the study of “metabolism” from the cell to the whole body. Core missions of the MET core will be to develop a new central MET core facility for pre-clinical Models (rodents) that provides a host of key measurements, allowing for thorough metabolic phenotyping. These approaches will include expertise and equipment to quantify real time measures of in-vivo energy metabolism, substrate metabolism, food intake regulation, and glucose/insulin tolerance testing along with non-invasive measures of body composition in pre-clinical rodent models (rat and mice). A second core feature of the MET core will be to provide resources to investigators to quantify mitochondrial energetics in cells and tissues from both rodent and human samples. Finally, the MET core will also have the capability to perform metabolic isotope methodologies (isotopic labeled glucose, fatty acids, etc.) for use in humans or rodent models (in-vivo or ex-vivo) to quantify overall metabolic flux. In summary, the MET core services will be organized to provide state-of-the art core services to investigators studying metabolism in pre-clinical rodent model systems and to translational measures of mitochondrial energetics and stable metabolic isotopes for use in cells, tissues, rodents, and human participants. The MET core is also designed to largely parallel the capabilities of the Human Energy Balance core so that scientists in the KC-MORE can conduct translational studies in both rodents and humans.

代谢核心：项目摘要代谢在肥胖的发展和肥胖的病理后果中起着基本作用导致慢性疾病。能量平衡失调和底物存储或氧化受损或氧化是发展肥胖和代谢疾病的基本特征。代谢失调均由两者驱动细胞线粒体功能的改变和全身代谢稳态的破坏。所以，将细胞线粒体能量与整个身体连接到体内底物代谢的代谢研究能量支出对于理解代谢功能障碍，肥胖和肥胖相关疾病状态。新陈代谢（MET）核心将为堪萨斯州的新陈代谢中心和肥胖研究（KC-more）具有专业知识，方法和设备，用于研究“代谢” 整个身体的细胞。大都会核心的核心任务将是开发一个新的中央大都会核心设施临床前模型（啮齿动物）提供了许多关键测量，可以彻底代谢表型。这些方法将包括量化体内实时度量的专业知识和设备能源代谢，底物代谢，食物摄入调节和葡萄糖/胰岛素耐受性测试在临床前啮齿动物模型（大鼠和小鼠）中的身体组成无创测量。第二个核心 MET核心的特征是向研究人员提供资源以量化细胞中的线粒体能量以及来自啮齿动物和人类样品的组织。最后，MET核心也将具有执行的能力代谢同位素方法（同位素标记的葡萄糖，脂肪酸等）用于人类或啮齿动物模型（体内或前体）来量化总体代谢通量。总而言之，MET核心服务将组织到向研究前临床前啮齿动物模型系统中研究代谢的研究人员提供最先进的核心服务并翻译了线粒体能量和稳定的代谢同位素的测量，用于细胞，组织，啮齿动物和人类参与者。 MET核心还旨在在很大程度上与人类的能力相似能量平衡核心使KC-More中的科学家可以在啮齿动物和人类。