Transgenic mice: generation and cryopreservation

转基因小鼠：生成和冷冻保存

• 批准号: 7153593
• 负责人: Oksana Gavrilova
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7153593
• 关键词: animal colony; biotechnology; cryopreservation; embryo /fetus preservation; genetically modified animals; laboratory mouse

Genetically modified mice contribute greatly to a better understanding of molecular mechanism underlying normal physiology and disease. Transgenic Core Facility generates transgenic mice by microinjection of foreign DNA into pronucleus of one-day embryo, performs rederivation of mouse lines and cryopreservation of mouse embryos. This year we have produced 7 new transgenic lines, rederived 20 lines and cryopreserved 16 mouse lines. We have also set up several new procedures, including reconstitution of mouse lines from frozen embryos, sperm collection and freezing, in vitro fertilization for rescue and rapid expansion of mouse colonies, and cryopreservation of embryos following in vitro fertilization.

转基因小鼠极大地有助于更好地理解正常生理和疾病的分子机制。转基因核心设施通过将外源DNA显微注射到一日胚胎的原核中来产生转基因小鼠，进行小鼠品系的再衍生和小鼠胚胎的冷冻保存。今年我们培育了 7 个新的转基因品系，再衍生 20 个品系和冷冻保存的 16 个小鼠品系。我们还建立了一些新程序，包括从冷冻胚胎中重建小鼠系、精子采集和冷冻、用于拯救和快速扩增小鼠群体的体外受精，以及体外受精后胚胎的冷冻保存。