CORE--TRANSGENIC ANIMAL MODEL DEVELOPMENT

核心--转基因动物模型开发

• 批准号: 6948119
• 负责人: Warren C LADIGES
• 金额: $ 15.66万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6948119
• 关键词: aging; animal colony; biological models; biomedical facility; biotechnology; cryopreservation; genetic manipulation; genetically modified animals; laboratory mouse; model design /development; transfection /expression vector

SPECIFIC AIMS 1. Continue to develop new genetically altered animal models for studying mechanisms of the biology of aging. 2. Provide a resource that enables University of Washington Health Sciences faculty involved in various aging projects to utilize genetic engineering technology for answering basic questions about the biology of aging. 3. Provide a resource and expertise in the construction of targeting vectors, injectable DNA constructs and inducible promoters systems for generating genetically altered animal models of the biology of aging. 4. Accelerate and enhance cryopreservation of embryos and sperm from useful genetically altered mouse models of aging for repository storage and conservation of housing resources. 5. Provide a training resource for fellows and junior faculty in transgenic and embryonic stem cell technology.

具体目标 1. 继续开发新的基因改造动物模型，用于研究衰老生物学机制。 2. 提供资源，使参与各种衰老项目的华盛顿大学健康科学系教师能够利用基因工程技术来回答有关衰老生物学的基本问题。 3. 提供构建靶向载体、可注射 DNA 构建体和诱导启动子系统的资源和专业知识，以生成衰老生物学的基因改变动物模型。 4. 加速和加强对来自有用的基因改造小鼠衰老模型的胚胎和精子的冷冻保存，用于储存库储存和住房资源保护。 5. 为转基因和胚胎干细胞技术领域的研究员和初级教师提供培训资源。