Metabolic Physiology and Energy Balance Core

代谢生理学和能量平衡核心

基本信息

批准号：
10586204
负责人：
BARBARA B. KAHN
金额：
$ 18.35万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-05-15 至 2028-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10586204
关键词：
Adipocytes Adipose tissue Affect Area Biological Body Composition Body Temperature Body Weight decreased Body measure procedure Bone Density Boston Carbon Dioxide Cell model Cells Communities Continuous Glucose Monitor Diabetes Mellitus Dose Dual-Energy X-Ray Absorptiometry Eating Endocrinology Ensure Equipment Fatty Acids Feedback Genes Genetic Vectors Genomics Glucose Goals Grant Homeostasis In Vitro Indirect Calorimetry Insulin Insulin Resistance Knock-in Lipids Lipolysis Measurement Measures Metabolic Metabolism Mission Modeling Mus Obesity Oxygen Consumption Pathway interactions Phenotype Physical activity Physiology Production Proteomics Quality Control Reproducibility Research Research Design Research Personnel Resolution Rodent Model Role Services Surveys Techniques Technology Training and Education Triglycerides United States National Institutes of Health Viral Vector Weight Gain awake blood glucose regulation design energy balance experience experimental study gene product glucose metabolism glucose transport glucose uptake in vivo insulin sensitivity knockout gene lipid metabolism member metabolic rate metabolomics mouse model overexpression respiratory response tool

项目摘要

METABOLIC PHYSIOLOGY AND ENERGY BALANCE CORE Project Summary/Abstract The main function of the Metabolic Physiology and Energy Balance Core is to perform experimental services to investigate alterations of glucose homeostasis, insulin sensitivity, lipid metabolism and body composition in rodent models of obesity and insulin resistance. Increasingly, the biologic roles of gene products and pathways emerging from genomic, proteomic, metabolomic and other discovery approaches are being queried by generating overexpression, gene knockout or knock-in of altered genes in rodent models. Thus, there is a growing need for characterization of new mouse models created by genetic or viral vector technologies. The Metabolic Physiology and Energy Balance Core is designed to meet the needs for characterization of rodent models that have alterations in glucose homeostasis, insulin action, and/or body composition and adiposity. The specific objectives of the Core are to carry out and interpret experiments for Core users aimed at investigating glucose homeostasis and energy balance. In vivo studies in awake mice include continuous glucose monitoring, measuring metabolic rates and energy balance by indirect calorimetry and body composition by EchoMRI and DEXA. Studies in primary adipocytes or adipose explants include glucose transport, metabolism, and lipolysis with assessment of insulin sensitivity. Services also include the differentiation of stromovascular cells into adipocytes and assessment of glucose uptake and insulin sensitivity. There is high demand for these services and we anticipate high utilization. The Core is actively utilized by BADERC members and other investigators in the Boston area. It enables investigators from other fields to carry out studies in the area of diabetes and metabolism that may not otherwise be feasible. Since the inception of the Core, we have received an increasing number of requests for services from investigators new to the field of metabolism, diabetes and obesity. The Core provides critical support for NIH grants for both new and established investigators. In many cases, the Core provides the tools that enable experts in other fields to apply their sophisticated techniques to metabolism research.

代谢生理和能量平衡核心项目概要/摘要代谢生理与能量平衡核心的主要功能是进行实验服务研究啮齿动物葡萄糖稳态、胰岛素敏感性、脂质代谢和身体成分的变化肥胖和胰岛素抵抗模型。基因组、蛋白质组、代谢组学中出现的基因产物和途径的生物学作用越来越多。和其他发现方法正在通过产生过度表达、基因敲除或敲入来受到质疑啮齿动物模型中基因的改变。因此，对创建的新小鼠模型的表征的需求日益增长通过遗传或病毒载体技术。代谢生理学和能量平衡核心旨在满足需要对葡萄糖稳态、胰岛素作用和/或身体发生改变的啮齿动物模型进行表征成分和肥胖。核心的具体目标是为核心用户进行和解释实验，旨在调查葡萄糖稳态和能量平衡。清醒小鼠体内研究包括连续血糖监测、通过间接量热法测量代谢率和能量平衡，通过 EchoMRI 和 DEXA 测量身体成分。原代脂肪细胞或脂肪外植体的研究包括葡萄糖转运、代谢和脂肪分解胰岛素敏感性评估。服务还包括基质血管细胞分化为脂肪细胞以及葡萄糖摄取和胰岛素敏感性的评估。对这些服务的需求很高，我们预计利用率高。 BADERC 成员和波士顿地区的其他研究人员积极利用该核心。它使来自其他领域的研究人员在糖尿病和代谢领域进行研究，否则这些研究可能无法进行可行的。自核心推出以来，我们收到了越来越多的服务请求新陈代谢、糖尿病和肥胖领域的新研究人员。核心为 NIH 拨款提供关键支持对于新的和已建立的研究者来说。在许多情况下，核心提供了使专家能够从事其他领域的工具领域将其复杂的技术应用于代谢研究。