Metabolic Physiology Core

代谢生理学核心

基本信息

批准号：
7925277
负责人：
BARBARA B. KAHN
金额：
$ 35.15万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-01 至 2015-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7925277
关键词：
Address Adipocytes Adipose tissue Animal Model Animals Area Arts Biological Biological Assay Biopsy Body Composition Body Weight Bone Density Boston Cannulas Catheters Cell Nucleus Consultations Coulter counter Custom DEXA Data Development Diabetes Mellitus Dissection Doctor of Philosophy Eating Educational process of instructing Energy Metabolism Equipment Experimental Designs Fatty Acids Fatty acid glycerol esters Funding Genetically Engineered Mouse Glucose Glucose tolerance test Grant Homeostasis Hormones Human Hyperglycemia Hypoglycemia Hypothalamic structure In Vitro Individual Infusion procedures Injection of therapeutic agent Institutes Insulin Insulin Resistance International Intravenous Journals Knockout Mice Laboratories Lead Leptin Leptin resistance Letters Manuscripts Measurement Measures Metabolic Metabolism Methods Modeling Mus Neuropeptides Non-Insulin-Dependent Diabetes Mellitus Nonesterified Fatty Acids Obesity Operative Surgical Procedures Paper Peptides Peripheral Phosphorylation Physiology Postdoctoral Fellow Preparation Process Production Protocols documentation Publications Publishing Pyruvate Pyruvates Rattus Research Research Design Research Personnel Rodent Rodent Model Role STAT3 gene Sampling Services Signal Pathway Signal Transduction Skeletal Muscle Techniques Testing Thinness Time Tissue Transplantation Tissues Transgenic Mice Transgenic Organisms Triglycerides United States National Institutes of Health Variant Work Writing adenylate kinase blood glucose regulation design energy balance experience fatty acid metabolism gene discovery glucose production glucose transport glucose uptake implantation in vitro Assay in vivo insulin sensitivity insulin sensitivity/resistance insulin signaling insulin tolerance knockout gene lipid biosynthesis lipid metabolism meetings member metabolic abnormality assessment metabolomics mouse model overexpression research study response subcutaneous tool

项目摘要

The Animal Metabolic Physiology Core is designed to meet the needs for characterization of rodent models that have alterations in glucose homeostasis, insulin action, leptin action and/or body composition. Its main function is to teach and provide consultation on ex vivo and in vivo methods to investigate the mechanisms for metabolic alterations in genetically-manipulated mouse models and in dietary models of obesity/leanness or insulin resistance/sensitivity. The specific objectives of the Core are 1) to teach and 2) to provide consultation to investigators in designing, carrying out and interpreting in vitro and in vivo studies to investigate the action of insulin and hormones that regulate energy balance such as leptin; glucose homeostasis; lipid metabolism; and body composition; 3) The Core also performs a limited number of these studies on selected mouse or rat models created by or obtained by members of the Center. Services of the Core include ex vivo methods such as adipocyte isolation, and dissection and incubation of isolated skeletal muscles to study glucose transport, insulin signaling, leptin signaling, and glucose and fatty acid metabolism. In vivo methods assessing insulin action and glucose homeostasis include insulin tolerance test, glucose tolerance test, signaling assays in response to insulin injection or infusion, and measurement of glucose turnover, endogenous glucose production and glucose uptake by individual tissues during clamp studies. In vivo methods to investigate biological actions of orexigenic and anorexigenic hormones include measurement of food intake, energy expenditure and leptin signaling in peripheral tissues and hypothalamic nuclei. The Core also provides specialized equipment such as a DEXA scanner for non-invasive analysis of body composition and bone mineral density and a Coulter Counter for determining adipocyte number and size distribution. The Core Director, Barbara B. Kahn, MD, the Associate Core Director, Odile Peroni, PhD and the Research Associates who work with the Core have extensive experience with the metabolic and signaling assays offered by the Core. They are developing new assays to characterize additional aspects of integrated fuel metabolism and enerov homeostasis.

动物代谢生理核心旨在满足表征具有葡萄糖稳态，胰岛素作用，瘦素作用和/或身体组成改变的啮齿动物模型的需求。它的主要功能是就体内和体内方法进行教学和提供咨询，以研究遗传操纵小鼠模型的代谢改变以及肥胖/瘦弱或胰岛素抵抗/敏感性的饮食模型的机制。核心的具体目标是1）教学和2）向研究人员提供咨询，以设计，进行和解释体外和体内研究，以研究调节能量平衡（如瘦素）的胰岛素和激素的作用；葡萄糖稳态；脂质代谢；和身体成分； 3）核心还对中心成员创建或获得的选定鼠标或大鼠模型进行了有限的研究。核心的服务包括离体方法，例如脂肪细胞分离，以及分离的骨骼肌的解剖和孵育，以研究葡萄糖转运，胰岛素信号传导，瘦素信号传导以及葡萄糖和脂肪酸代谢。评估胰岛素作用和葡萄糖稳态的体内方法包括胰岛素耐受性测试，葡萄糖耐受性测试，响应胰岛素注射或输注的信号测定，以及葡萄糖更换，内源性葡萄糖的产生和培养基研究期间单个组织的葡萄糖摄取的测量。研究甲状腺素和厌食激素的生物学作用的体内方法包括测量食物摄入量，能量消耗和周围组织中的瘦素信号传导和下丘脑核。该核心还提供了专门的设备，例如DEXA扫描仪，用于非侵入性分析人体成分和骨矿物质密度，以及用于确定脂肪细胞数量和尺寸分布的Coulter计数器。核心主任，医学博士Barbara B. Kahn，Odile Peroni博士的副总监和与核心合作的研究助理具有丰富的经验，可以在核心提供的代谢和信号分析中。他们正在开发新的测定法，以表征综合燃料代谢和Enerov稳态的其他方面。