Molecular evolution of Theileria parasites in Southern Asia

南亚泰勒虫寄生虫的分子进化

基本信息

批准号：
10041150
负责人：
ONUMA Misao
金额：
$ 7.74万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

Bovine piropurasmosis due to Theileria sergenti is a major cause of economic loss in grazing cattle in Japan. T. sergentii / buffeli / orientalis grop is referred to as bengin theileria parasites of cattle and is known to be distributed all over the world. Previously, we developed the allele-specific polymerase chain reaction (PCR) which specifically amplifies lach allelic from using 3 sets of olgonucleotide primers designed from nucleotide sequences of major Immunodominant primers designed from nucleotide sequences of major Immunodominant piroplasm surface protein (p32) genes of two T. sergenti stocks, Ikeda (1) and Chitose c and one t, buffeli (B) stok, Warwick, and appled the method to the field survey of benign theileria parasites in southern asian countries.During 2 years, we have collected blood samples from the following countries : Korea (Cheju island), Thailand, Cambodia, India, Paklstan, Vietnam and China. Parasites DNA was extracted and the DNA was analyzed by using allele-specific PCR. In Korea (Cheju island), all the isolates contained I type parasites as well as C type parasites. However In Thailand and Cambodia, Major parasites distributed there countries were Thai type parasites, different from T. sergentii /buffeli / orientalis. C type parasites and T, buffeli type parasites were also isolated from Thailand and Cambodia. In India and Pakistan, major theileria parasites are known as T. annulata. We expected to isolate benign theileria parasites, however, we could not isolate benign theileria parasites. The present data showed that benign theileria parasites in east and Southern asian countries were mixed parasites populations.

由于Theileria sergenti引起的牛吡铂是日本放牧牛经济损失的主要原因。 T. sergentii / buffeli / orientalis抓球被称为牛的牛寄生虫，已知在世界范围内分布。 Previously, we developed the allele-specific polymerase chain reaction (PCR) which specifically amplifies lach allelic from using 3 sets of olgonucleotide primers designed from nucleotide sequences of major Immunodominant primers designed from nucleotide sequences of major Immunodominant piroplasm surface protein (p32) genes of two T. sergenti stocks, Ikeda (1) and Chitose c还有一个T，Buffeli（B）Stok，Warwick，并将该方法添加到南亚国家的良性Theileria寄生虫的现场调查中。在2年的时间里，我们从以下国家收集了血液样本：韩国（Cheju Island），泰国，泰国，泰国，坎巴迪亚，印度，印度，帕克斯坦，越南和中国。提取寄生虫DNA，并使用等位基因特异性PCR分析DNA。在韩国（Cheju Island），所有分离株都包含I型寄生虫以及C型寄生虫。然而，在泰国和柬埔寨，在那里分发的主要寄生虫是泰国型寄生虫，与塞金蒂（T. sergentii / buffeli / orientalis）不同。 C型寄生虫和T，Buffeli型寄生虫也从泰国和柬埔寨分离出来。在印度和巴基斯坦，主要的寄生虫被称为T. annulata。我们期望隔离良性的寄生虫，但是，我们无法分离良性的寄生虫。目前的数据表明，东亚和南亚国家的良性寄生虫是寄生虫种群混合的。