Development of anti-tick vaccine

抗蜱疫苗的研制

• 批准号: 12356008
• 负责人: ONUMA Misao
• 金额: $ 26.86万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12356008/
• 关键词: tick; anti-tick vaccine; recombinant antigen; Pathogens tramsmission inhibition; anti-blood feeding; serpin; ダニ抗原; ダニワクチン; カルレチクリン; 組み換え蛋白; マダニ; 節足動物媒介感染症; 組換え抗原

Maintenance of mammalian homeostatic balance is a multi-faced process involving an interaction of many proteins, however the need for a balance and coordinated interaction of many proteins, however the need for a balance and coordinated interaction between members of the serine proteinase super family and their inhibitors, known as serpins occupy a central role. In mammalian systems where extensive data exists, both serine proteinases and serpins are known to regulate such important functions as the blood coagulation cascade, fibrinolysis, food digestion, fertilization, inflammatory and immune responses. Imbalances or deficiencies in function of either serine proteinases or serpins have resulted in diseases. Ticks encode proteinases or serpins to disrupt the host homeostatic balance to their advantage.For this reason, we examined the molecular cloning of tick-encoded serpins as a candidate for tick vaccine. By using rapid amplification of the cDNA (RACE method), two genes (HLS l and HLS 2) from H. longicornis were cloned. Both recombinant HLS l and 2 showed the anti-coagulant activities. Vaccinaion of rabbits with either recombinant HLS 1 or 2 expressed in Escherichia coli resulted in significant anti-tick immunity.At present, we have characterized 4 tick salivary gland proteins whose recombinant products were effective against tick. We are planning to use these 4 cocktail vaccine for prevent the transmission of pathogens.

项目成果

Tsuda A.: "cDNA cloning, characterization and vaccine effect analysis of Haemaphysalis longicornis tick saliva proteins"Vaccine. 19. 4287-4296 (2001)

Tsuda A.：“长角血蜱唾液蛋白的cDNA克隆、表征及疫苗效果分析”疫苗。

Mulenga A.et al.: "Characterization of an 84 kDa protein inducing an immediate hypersensitivity reaction in rabbits sensitized to Haemaphysalis longicornis ticks."Biochemica et Biophysica Acta. 1501. 219-226 (2000)

Mulenga A. 等人：“84 kDa 蛋白质的表征，该蛋白质在对长角血蜱敏感的兔子中诱导立即超敏反应。”《生物化学与生物物理学学报》。

Mulenga A.: "Blood meal acquisition by tick ; Molecular advances and implication for vaccine development"Jpn. J. Vet. Res.. 49(4). 261-272 (2002)

Mulenga A.：“蜱虫获取血粉；分子进展及其对疫苗开发的影响”Jpn。

Nakajima M.: "Passive immunization with monoclonal antibodies : effects on Haemaphysalis longicornis tick infestation of BALB/c mice"Jpn. J. Vet. Res.. (in press). (2003)

Nakajima M.：“单克隆抗体被动免疫：对 BALB/c 小鼠长角血蜱感染的影响”Jpn。

Passive immunization with monoclonal antibodies : effects on Haemaphysalis longicornis tick infestation of BALB/c mice.

单克隆抗体被动免疫：对 BALB/c 小鼠长角血蜱感染的影响。

• 发表时间: 2003
• 期刊: Jpn. J. Vet. Res. 50(4)
• 作者: Nakajima M.