Immunological modulation and control of infections diseases by cytokaines

细胞因子对感染性疾病的免疫调节和控制

基本信息

批准号：
09356009
负责人：
ONUMA Misao
金额：
$ 20.22万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09356009/
关键词：
cytokine profile Th 1 and Th 2 immunoregulatory peptides cytokine and pathogesis 免疫抑制ペプチド競合PCR TNFα

项目摘要

In order to understand the role of cytokines in protective immune responses against bovine leukemia virus (BLV) infection, 12 sheep were inoculated with BLV and the levels of cytokine mRNA expressions in the peripheral blood mononuclear cells (PBMCs) were analyzed. The mRNA expressions of IFN γ, IL2, IL4 and IL10 in PBMCs were downregulated after BLV infection in all sheep. However, the expressions of tumor necrosis factor α (TNF α) mRNA was up-regulated only in BLV-resistant sheep in which BLV were eliminated, though down-regulated in -susceptible sheep in which BLV propagated. This may suggest that TNF α play an important role in the protection against BLV infection during the early phase of infection. Additionally, the mRNA expressions of TNF α receptors type I(TNFR I) and type II (TNFR II) were investigated in BLV-resistant and -susceptible sheep PBMCs.Interestingly, the mRNA expression of TNFR I was significantly downregulated only in the BLV-susceptible sheep. By the use of in vitro stimulation with TNF α, higher stimulation index was observed for PBMCs from BLV-susceptible than -resistant sheep. In addition, flowcytometric analysis showed that membrane form of TNF α, which is thought to be one of the ligands which induce B-cell activation, was expressed more on PBMCs from BLV-susceptible than those from -resistant sheep. These results suggest that TNFα and its receptors are involved in the development of BLV-induced B-cell proliferation in sheep.

为了了解细胞因子在针对牛白血病病毒（BLV）感染的保护免疫复杂中的作用，分析了12只绵羊与BLV接种，分析外周血单核细胞（PBMC）中细胞因子mRNA表达水平。在所有绵羊中，BLV感染后，PBMC中IFNγ，IL2，IL4和IL10的mRNA表达被下调。然而，仅在抗BLV的绵羊中上调了肿瘤坏死因子α（TNFα）mRNA的表达，尽管BLV在blv繁殖的可敏感的绵羊中被下调，但在其中消除了BLV的绵羊。这可能表明TNFα在感染的早期阶段在防止BLV感染的保护中起重要作用。此外，在BLV耐药和 - 敏感的绵羊PBMC中研究了TNFα受体（TNFR I）和II型（TNFR II）的mRNA表达。在抗BLV耐药和 - 敏感的绵羊PBMCs中，TNFR I的mRNA表达仅在BLV敏感的sheep中仅在BLV-seep中显着下降。通过将体外刺激与TNFα一起使用，观察到来自BLV的PBMC比耐药的绵羊观察到更高的刺激指数。此外，流经术分析表明，被认为是诱导B细胞激活的配体的TNFα的膜形式在BLV吸引的PBMC上比耐药的绵羊的PBMC表达了更多。这些结果表明，TNFα及其受体参与BLV诱导的绵羊B细胞增殖的发展。