Molecular mechanisms of diversity of theileria parasite genes and control of the disease

泰勒虫寄生虫基因多样性的分子机制及其疾病控制

基本信息

批准号：
11460141
负责人：
ONUMA Misao
金额：
$ 9.79万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

Theileria sergenti is a tick-borne protozoa of cattle. Benign bovine theileria parasites, which are presumed to be of the T.sergenti/buffeli/orientalis group are distributed in Asian countries. The parasites have the major piroplasm surface protein (MPSP) gene which is one of the means of parasite evasion of host immunity. T.sergenti has 4 chromosomes similar to T.parva.Most of genes used for molecular karyotype showed a conserved synteny between T.sergenti and T.parva.To underatand the genome mapping of theileria parasites, characterization of bovine genes expressed in T.parva transformed T-lymphoblastoid cell lines was performed by using expressed sequence tag (EST) sequencing approach of random cDNA clones from libraries. Single pass sequencing was done on 394 cDNA clones and the sequences search for significant homolgies in GeneBank, EBML and DDBJ databases. About 141 clones showed significant homology to genes in the databases. The majority of these ESTs (47) showed homology to human sequences and only a few (19) matched bovine sequence. 126 clones did not match database sequences and may represent novel transcripts.The EST sequencing approach has recently contributed tremendously to discover of new genes in several parasites. Thus, we performed the sequencing of 461 cDNA clones from the schizont stage of T.parva. The cDNA colnes were mapped to the 4-T.parva chromosomes and 20 of these clones were mapped to the 33 Sfil fragments of the T.parva genome. EST sequencing demonstrated its potential in the identification of new genes in this stage of the parasite and has contributed greatly to the addition of ESTs into the T.parva database. The sequence data reported is invaluable for T.parva genome mapping and in the comparative genome mapping of theileria parasites.

Theileria Sergenti是牛tick虫的原生动物。良性的牛寄生虫寄生虫被认为是t.sergenti/buffeli/orientalis组的良性寄生虫。寄生虫具有主要的雌性表面蛋白（MPSP）基因，这是寄生虫免疫的寄生虫的一种手段之一。 t.sergenti具有与t.parva相似的4个染色体。用于分子核型的大多数基因表明，T.Sergenti和T.Parva.在底膜下进行了保守的同步，并在寄生虫的基因组映射下，使用T.Parva序列表达的t.parva sequiners trancients trancients trecients parva terienting parva termities transing trecorting tryme terienting trecting tryme eTRINCTION（parva）的基因组映射。来自库的随机cDNA克隆的方法。单个通过测序是在394个cDNA克隆上进行的，序列在Genebank，EBML和DDBJ数据库中搜索了显着的同源。大约141个克隆与数据库中的基因显示出显着的同源性。这些EST（47）中的大多数与人类序列显示同源，只有少数（19）个匹配的牛序列。 126个克隆与数据库序列不匹配，并可能代表新的转录本。EST测序方法最近为在几种寄生虫中发现新基因做出了巨大贡献。因此，我们从T.Parva的Schizont阶段对461个cDNA克隆进行了测序。将cOLNE映射到4-T.PARVA染色体上，其中20个克隆被映射到T.Parva基因组的33个SFIL片段。 EST测序证明了其在寄生虫的这一阶段鉴定新基因的潜力，并为将EST添加到T.Parva数据库中做出了巨大贡献。报道的序列数据对于T.Parva基因组映射以及Theileria寄生虫的比较基因组映射是无价的。