Genetic diversity of bovine lentivirus distributed in south Asian countrirs

南亚国家牛慢病毒遗传多样性

基本信息

批准号：
15405035
负责人：
ONUMA Misao
金额：
$ 9.02万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

To understand the genetic diversity of bovine lentivirus (BIV), we have examined serological survey of BIV infection in cattle in Hokkaido and found 10% of positive reactors. From the seropositive animals, 3 BIV were isolated and DNA samples were obtained from peripheral blood lymphocytes of these positive animals for the detection of BIV proviral DNA by nested-PCR to detect a part of the pol gene. A 298-bp pol region fragment specific to BIV proviral DNA was amplified. Nucleotide sequences of the amplified fragments from Japanese BIV field isolates had 99% homology with American isolate, R-29. Amplification of the C2, V1, V2 and C3 regions of the env gene of Japanese BIV isolates were carried out by nested PCR. All 3 isolates seemed to be smaller, several base substitutions were observed in the V1 region and deletions were found in the V2 region of the env gene when compared to that of American R-29 isolate.In Cambodia, samples from 544 cattle and 43 water buffalo were obtained and BIV seropositive rate were 26% in cattle and 17% in water buffalo. Five BIV proviruses were obtained from seropositive animals. Nucleotide sequence of pol region was highly conserved when compared to that of American and Japanese BIV. In contrast, env region of Cambodian BIV field isolates seemed to be smaller than those of American isolates. Nucleotide substitutions which alter amino acid sequences were observed in the V2 region of Cambodian isolates.Total 262 serum samples were obtained from cattle in Zambia and seropositive rate was 11.4%. Four BIV proviruses were obtained by nested PCR. Nucleotide sequences of pol region of these proviruses were highly homologous (over 97%) to that of American and Japanese isolates.

为了了解牛慢病毒（BIV）的遗传多样性，我们检查了北海道牛的BIV感染的血清学调查，发现了10％的阳性反应堆。从血清阳性动物中，分离出3个BIV，并从这些阳性动物的外周血淋巴细胞中获得DNA样品，通过嵌套-PCR检测BIV前病毒DNA，以检测一部分POL基因。扩增了针对BIV病毒DNA的298 bp POL区域碎片。来自日本BIV场分离株的扩增片段的核苷酸序列与American Enaline，R-29具有99％的同源性。日本BIV分离株的ENV基因的C2，V1，V2和C3区域的扩增是通过嵌套PCR进行的。与美国R-29分离株相比，在V1区域中观察到所有3个分离株，在V1区域中观察到了几个基本取代，在Env Gene的V2区域中发现了缺失。在柬埔寨，在牛和17％的柬埔寨获得了544个牛和43个水牛的样品，并在牛中获得了26％。从血清阳性动物中获得了五个BIV原病毒。与美国和日本BIV相比，POL区域的核苷酸序列高度保守。相比之下，柬埔寨BIV场分离株的Env区域似乎比美国分离株小。在柬埔寨分离株的V2区域中观察到改变氨基酸序列的核苷酸取代。总体262血清样品是从赞比亚的牛获得的，血清阳性速率为11.4％。通过嵌套PCR获得了四个BIV预科病毒。这些原病毒的POL区域的核苷酸序列与美国和日本分离株的核苷酸序列高度同源（超过97％）。

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Usui T., et al.: "Protective effects vaccination with bovine leukemia virus (BLV) tax DNA against BLV infection in Sheep"J.Vet.Med.Sci.. 65(11). 1201-1205 (2003)

Usui T.等人：“用牛白血病病毒（BLV）对DNA进行疫苗接种以对抗绵羊BLV感染的保护作用”J.Vet.Med.Sci. 65(11)。

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0
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Konnai S., et al.: "Sequences and diversity of 17 new Over-DRB1 alleles from three breeds of sheep"Eurp.J.Immunogenetic.. 30. 275-282 (2003)

Konnai S.等人：“来自三种绵羊品种的 17 个新 Over-DRB1 等位基因的序列和多样性”Eurp.J.Immunogenic.. 30. 275-282 (2003)

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Evidence for the presence of bovine immunodeficiency virus in cattle in Turkey

土耳其牛体内存在牛免疫缺陷病毒的证据

DOI：
发表时间：
2003
期刊：
Jpn. J. Vet. Res. 51(1)
影响因子：
0
作者：
Konnai S;Onuma M;et al.;Meas S.et al.;Meas S.et al.;小沼操;Usui T.et al.;Meas S.et al.
通讯作者：
Meas S.et al.

Protective effects vaccination with bovine leukemia virus (BLV) tax DNA against BLV infection in Sheep

牛白血病病毒 (BLV) 疫苗接种对绵羊 BLV 感染的保护作用