Search for biological active molecules from tick salivary glands

从蜱唾液腺中寻找生物活性分子

• 批准号: 15380206
• 负责人: ONUMA Misao
• 金额: $ 9.73万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15380206/
• 关键词: anti-tick vaccine; tick saliva proteins; anti-coagulant; Haemaphysalis tick; Immunosuppression; ダニ; Haemaphysalis longicornis; 遺伝子解析

To obtain biological active molecules from tick, a cDNA library was constructed from semi-fed female Haemaphysalis longicornis salivary glands. Randomly selected clones were sequenced and total of 653 sequences were analyzed by bioinformatic programs including NCBI blastX. The sequences were assembled into 321 non-redundant clusters. About 35 % of the sequences showed significant similarity to known genes in homology search against non-redundant protein database and suggested as function predictable genes whereas rest of the 65 % had no homologs. Two thirds of the function predictable sequences were suggested to be so-called house keeping genes. Among non-house keeping genes, function annotated genes which we found were as follows ; several protease inhibitors, two metalloproteases, anti-thrombin type coagulation inhibitors previously identified from H. longicpornis and a homolog of D.andersoni derived predicted immunosuppressant protein (Da-p36). Recombinant of homolog protein of Da-p36 showed an immunosuppressive activity against bovine lymphocytes. These proteins may play important roles during tick feeding and are expected to be novel tick vaccine antigen candidates.

为了从tick获得生物活性分子，由半喂养的雌性血囊乳核乳腺唾液腺构建一个cDNA文库。对随机选择的克隆进行测序，并通过包括NCBI BLASTX在内的生物信息学计划分析了总共653个序列。将序列组装成321个非冗余簇。大约35％的序列与非冗余蛋白数据库中的同源性搜索中的已知基因显示出显着的相似性，并建议作为可预测基因的功能，而65％的其余基因没有同源物。提示函数的三分之二可预测的序列是所谓的房屋保留基因。在饲养基因的非房屋中，我们发现的功能注释的基因如下；几种蛋白酶抑制剂，两种金属蛋白酶，抗凝血酶型凝血抑制剂先前从远程杆菌中鉴定出的抗凝血蛋白抑制剂以及d.andersoni衍生的预测免疫抑制剂蛋白的同源物（DA-P36）。 DA-P36同源蛋白的重组表现出针对牛淋巴细胞的免疫抑制活性。这些蛋白质可能在滴答喂食过程中起重要作用，预计将是新颖的tick疫苗抗原候选物。

项目成果

Nakajima M., et al.: "Production and characterization of monoclonal antibodies against midgnt of ixodid tick Haemaphysalis longicornis"Vet.Parasitol.. 115. 355-363 (2003)

Nakajima M.等人：“针对长角血蜱中的蜱虫的单克隆抗体的生产和表征”Vet.Parasitol.. 115. 355-363 (2003)

Cloning, expression and partial characterization of a Haemaphysalis longicornis and a Rhipicephalus appendiculatus glutathione S-transferases.

长角血蜱和附肢扇头蜱谷胱甘肽 S-转移酶的克隆、表达和部分表征。

• 发表时间: 2004
• 期刊: Insect Biochem. Mol. Biol. 13
• 作者: da Silva Vaz.

Passive immunization with monoclonal antibodies : effects on Haemaphysalis longicornis tick infestation of BALB/c mice.

单克隆抗体被动免疫：对 BALB/c 小鼠长角血蜱感染的影响。

• 发表时间: 2003
• 期刊: Jpn. J. Vet. Res. 50(4)
• 作者: Nakajima M.

Production and characterization of monoclonal antibodies against midgnt of ixodid tick Haemaphysalis longicornis.

针对长角血蜱中蜱的单克隆抗体的产生和表征。

• 发表时间: 2003
• 期刊: Vet. Parasitol. 115
• 作者: Nakajima M.

ダニの吸血を阻害する抗ダニワクチンの開発

开发抑制蜱吸血的抗螨疫苗

• 发表时间: 2004
• 期刊: 獣医畜産新報 57(9)
• 作者: 小沼 操 ら