Patho-physiological study of piloplasmosis in domestic animals

家畜毛虫病的病理生理学研究

• 批准号: 05454122
• 负责人: MAEDE Yoshimitsu
• 金额: $ 4.22万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454122/
• 关键词: Piroplasm; Babesia; Erythrocyte; Anemia; イヌ

The mechanisms of destruction of nonparasitized erythrocytes and thrombo-cytopenia in dogs infected with Babesia gibsoni were studied. The results were as follows.1. The membrane proteins from erythrocytes of the infected dogs showed a markedly reduced level of band 4.1a compared to that from normal dogs. The concentrations of both cholesterol and phospholipids in the erythrocyte menbranes from the infected dogs were higher than those from normal dogs.2. When B.gibsoni was cultured together with erythrocytes, marked increase of methemoglobin (metHb) and malondialdehyde concentrations of erythrocytes in the cultures were observed together with high multiplication of the parasites, but not at low multiplication of the parasites in the culture. When erythrocytes from the infected dogs were incubated with acetylphenyl-hydrazine, the metHb formation in erythrocytes was much higher at day 3 than at day 0 after the inoculation of the parasites.3. Addition of erythrocytes or the supernatant from B.gibsoni-infected erythrocyte culture to canine platelet-rich plasma showed enhanced platelet aggregation with ADP.These results indicate that nonparasitized erythrocytes of dogs infected with B.gibsoni are sensitive to oxidative stress, and that the factor which promoted canine platelet aggregation may be associated with thrombocytopenia observed in canine babesiosis.

研究了感染了babesia gibsoni的狗中非寄生的红细胞和势头细胞减少症的破坏机制。结果如下1。与正常狗相比，感染犬红细胞的膜蛋白显示出4.1a的水平明显降低。感染犬的红细胞蒙植物中胆固醇和磷脂的浓度高于正常狗的浓度。2。当B.Gibsoni与红细胞一起培养时，观察到培养物中红细胞（MECHB）明显增加和丙二醛浓度的浓度与寄生虫的高倍数一起观察到，但在培养物中寄生虫的低繁殖症中没有观察到。当将来自感染犬的红细胞与乙酰苯基氢嗪一起孵育时，在寄生虫接种后的第3天，红细胞中的甲虫形成要高得多。3。添加红细胞的红细胞或来自犬富含血小板血浆的红细胞培养物中的红细胞培养物与ADP的血小板聚集增强。这些结果表明，非寄生的狗的非寄生性红细胞与B.Gibsoni感染的狗可以与该因素促进癌症，并具有促进罐头的因素，并具有促进性罐头的因素，并具有促进性罐头的因素。在犬只babesiosis中。

项目成果

MURASE,T., IWAI,M.& MAEDE,Y.: "Direct evidence for preferential multiplication of Babesia gibsoni in young erythrocytes" Parasitol Res. 79. 269-271 (1993)

村濑 T.、岩井 M.

Murase,T.: "Direct evidence for preferential multiplication of Babesia gibsoni in young erythrocytes" Parasitol Res. 79. 269-271 (1993)

Murase,T.：“吉氏巴贝虫在年轻红细胞中优先增殖的直接证据”Parasitol Res。

Murase,T.: "Oxidative damage and enhanced erythrophagocytosis in canine erythrocytes infected with Babesia gibsoni" Parasitol Res. (発表予定).

Murase, T.：“感染吉氏巴贝虫的犬红细胞中的氧化损伤和增强的噬红细胞作用”Parasitol Res。

Murase,T.: "Mechanism of thrombocytopenia in dogs infected with Babesia gibsoni" Am J Vet Res. (発表予定).

Murase, T.：“感染吉布氏巴贝虫的狗的血小板减少机制”Am J Vet Res。