Culture of piroplasms using some components of reticulocytes for the purpose of cell-free culture of the parasite.

使用网织红细胞的某些成分培养梨质体，以实现寄生虫的无细胞培养。

• 批准号: 06556049
• 负责人: MAEDE Yoshimitsu
• 金额: $ 7.55万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06556049/
• 关键词: Babesia; reticulocyte; culture; glutamate; ピロプラズマ; タイレリア; Babesia; reticulocyte; culture; glutamate; ピロプラズマ; タイレリア

To clarify the factors which enhance the multiplication of Babesia gibsoni in canine reticulocytes in vitro.1. Babesia parasites were cultured together with canine erythrocytes with hereditary high concentration of glutamate, or cells with low glutamate.2. Babesia parasites were cultured in the medium containing several materials which present in reticulocytes.(Results)1. The highest multiplication of the parasites was observed in the erythrocytes containing high glutamate.2.(1) A high multiplication of the parasites was observed when the parasites were cultured with glucose or glutamate.(2) The multiplication of the parasites was increased when the parasites were cultured together with resealed cells containing sediments of hemolysate of reticulocytes.(Conclusion)These results indicate that both glutamate and glucose are necessary for growth of B.gibsoni, and that the intracellular organelles may be an important factor which enhance the multiplication of B.gibsoni in canine reticulocytes.

为了阐明增强犬鳞状细胞在体外网状细胞中Babesia gibsoni繁殖的因素。1。将贝贝西亚寄生虫与遗传性高浓度谷氨酸或低谷氨酸的细胞一起培养。2。在含有网状细胞中存在的几种材料的培养基中培养贝贝斯寄生虫。（结果）1。在含有高谷氨酸的红细胞细胞中观察到寄生虫的最高繁殖。结果表明，谷氨酸和葡萄糖对于b.gibsoni的生长都是必需的，并且细胞内细胞器可能是增强犬网状细胞中b.gibsoni繁殖的重要因素。