Molecular epidemiology of Theileria infections in Africa : Characterization of parasites of wild and domestic animals.

非洲泰勒虫感染的分子流行病学：野生和家养动物寄生虫的特征。

基本信息

批准号：
07041119
负责人：
SUGIMOTO Chihiro
金额：
$ 5.31万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07041119/
关键词：
Theileria homoprotozoa Africa wild animal PCR rRNA gene Babesia Piroplasm 分子疫学遺伝子進化野性動物原生動物

项目摘要

Lymphoblastoid cell lines infected with Theileria parva schizonts were established from cattle in Central Province of Zambia. The parasites were genetically and antigenically different from buffalo-derived T.parva (T.parva lawrencei) and also from cattle-derived parasites (T.parva parva) in Eastern Province of Zambia, as revealed by Southern blot analysis and indirect fluorescent assay by using a panel of anti-schizont monoclonal antibodies. Polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) analysis of major piroplasm and shizont protein genes of Zambian isolates also confirmed the above-mentioned results.DNA samples were prepared from Theileria-infected African buffalo in South Africa and analyzed for the detection of Theileria by PCR based on small subunit ribosomal RNA (srRNA) gemes. They contained several species of Theileria, including T.buffeli (or sergenti) and other parasites unique in this animal species. Possible transmission of the former sp … More ecies among cattle and buffaloes are suggested.A major piroplasm surface antigen gene of Theileria parasite isolated from a sable antelope was amplified by PCR and its nucleotide sequence was determined. The parasite was phylogenically distinct from other Theileria species isolated from cattle and African buffalo.Babesia-like piroplasms were detected in erythrocytes of zebra in South Africa, and successfully cultured in vitro. They were morphologically similar to Babesia equi in horses. Further molecular characterizations of the zebra-derived parasites are now underway based on EMA-1 gene analysis.Several other piroplasms (Babesia or Theileria) were also detected in blood smears from other wild animal species including kudu, cheetah, giraffe, lion.caracol (wild cat in Africa). Some of the parasites have not been classified so that further molecular characterizations of the parasites based on srRNA genes are essential to clarify taxonomical and phylogenical relationships among parasites from wld animals and those from domestic animals. Less

从赞比亚中部省的牛建立了感染Theileria parva schizonts的淋巴细胞细胞系。这些寄生虫通常与抗原源不同于源自水牛的T.Parva（T.Parva Lawrencei），以及来自牛源的寄生虫（T.Parva Parva）的赞比亚省，通过使用Anti-Schiz的anti-Schiz antibibibibibibibibibibibibibibibibibibibes揭示，在赞比亚东部省份的牛源性寄生虫（T.Parva Parva）。 Polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) analysis of major pyroplasm and shizont protein genes of Zambian isolates also confirmed the above-mentioned results.DNA samples were prepared from Theileria-infected African buffalo in South Africa and analyzed for the detection of Theileria by PCR based on small subunit ribosomal RNA (srRNA) gemes.他们包含几种theileria，包括T. buffeli（或Sergenti）和该动物物种中独特的其他寄生虫。建议在牛和水牛中越来越多地传播。通过PCR扩大了从黑貂羚羊分离的寄生虫的主要寄生虫寄生虫的主要表面抗原基因，并确定了其核苷酸序列。该寄生虫在系统上与从牛和非洲水牛分离的其他细菌物种不同。在南非的斑马的红细胞中检测到类似Babesia样的piroplasms，并在体外成功培养。它们在形态上类似于马匹在马中的比较。现在，基于EMA-1基因分析，还进行了斑马衍生的寄生虫的进一步分子特征。在其他野生动物物种（包括Kudu，Cheetah，Cheetah，Giraffe，Giraffe，Lion.caracol.caracol.caracol.caracol.caracol.caracol.caracol.caracol.caracol.caracol.caracol.caracol.caracol）的血液涂片中，还检测到其他其他脊髓裂谷（Babesia或Theileria）。某些寄生虫尚未分类，因此基于SRRNA基因的寄生虫的进一步分子特征对于对WLD动物和家畜的寄生虫之间的分类学和系统生态关系进行分类至关重要。较少的