Genome analysis of Theileria orientalis and its functional genomics

东方泰勒虫基因组分析及其功能基因组学

• 批准号: 17208026
• 负责人: SUGIMOTO Chihiro
• 金额: $ 31.2万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17208026/
• 关键词: Genome; Veterinary Medicine; Microbe; Infectious disease; Protozoa; タイレリア; ショットガン解析; cDNA

The complete genome sequence of Theileria orientalis, the causal agent of bovine theileriosis in Japan, was determined. Total genome size is 8,983,596 bp consisting of four chromosomes. Two full-length cDNA libraries from intraerythrocytic piroplasm were constructed by using oligo-capping and vector-trapper methods and conventional cDNA library stage. A total of 27,478 ESTs were mapped on the genome sequence. By using two gene prediction soft-wares, Glimmer 2 and Genewise, about 3,900 genes were predicted, which were subsequently automatically and manually annotated. By comparison of T. orientalis genome with those of T. parva and T. annulata revealed significant structural differences. Among all, T. orientalis contains single copies of genes which are found as tandem-repeated multiple copy gene families in T. parva/annulata, and structures at subtelomeric ends of each chromosome are different.By synteny analysis, orthologues of T. parva/annulata schizont and spolozoite proteins were identified. Furthermore, orthologue of T. orientalis band 3 binding protein (TpSCOP) was found and its actin-binding activity and possible role in host cell resistance apoptosis were determined.

确定了日本的牛theerieriosis的因果剂Theileria Orientalis的完整基因组序列。总基因组大小为8,983,596 bp，由四个染色体组成。通过使用寡聚式限制和载体 - 捕获器方法和常规cDNA库阶段构建了来自肠内恐惧质质质中的两个全长cDNA文库。在基因组序列上映射了总共27,478个EST。通过使用两个基因预测软孔，闪光2和Genewise，预测了约3,900个基因，随后自动并手动注释。通过将东方基因组与帕尔瓦（T. parva）和annulata的T. parva基因组进行比较，发现了显着的结构差异。其中，Orientalis T. capies含有单个基因副本，这些基因被发现是T. parva/annulata中的串联多个复制基因家族，每个染色体的亚近端末端的结构都是不同的。通过同步分析，parva/annulata schizots and annulata schizont和spolozoite and spolozoite and spolozoite prote protied protientied。此外，发现了Orientalis带3结合蛋白（TPSCOP）的直系同源物，并确定了其肌动蛋白结合活性，并确定了在宿主细胞耐药性凋亡中的作用。

项目成果

Theileria parvaシゾントステージにおけるToMRPホモログ蛋白質の発現

ToMRP同源蛋白在细小泰勒虫裂殖期的表达

• 发表时间: 2005
• 作者: 林田;京子
• 通讯作者: 京子

Theileria parvaのシゾント期において発現されるTpSCOPはアクチンへの結合性を有する

细小泰勒虫裂殖期表达的 TpSCOP 具有肌动蛋白结合特性

• 发表时间: 2007
• 作者: Thekisoe;O.;工藤京子
• 通讯作者: 工藤京子

Rapl signal controls B cell receptor repertoire and generation of self-reactive Bla cells.

Rapl 信号控制 B 细胞受体库和自身反应性 Bla 细胞的生成。

• 发表时间: 2006
• 期刊: Immunity 24(4)
• 作者: Ishida;D.
• 通讯作者: D.

A trypanosome species isolated from naturally infected Haemaphysalis hystricis ticks in Kagoshima Prefecture

从鹿儿岛县自然感染的猪血蜱蜱中分离出的锥虫物种

• 发表时间: 2007
• 期刊: Japan., Parasitology 134(Pt 7)
• 作者: Thekisoe;O.M.
• 通讯作者: O.M.

Cloning and characterization of a ribosomal protein L23a from Haemaphysalis qinghaiensis eggs by immuno screening of a cDNA expression library

• DOI: 10.1007/s10493-007-9065-2
• 发表时间: 2007-04-01
• 期刊: EXPERIMENTAL AND APPLIED ACAROLOGY
• 影响因子: 2.2
• 作者: Gao, Jinliang;Luo, Jianxun;Yin, Hong
• 通讯作者: Yin, Hong