Molecular epidemiological study on blood-borne protozoan parasites of small ruminants in China

我国小反刍动物血源性原虫寄生虫的分子流行病学研究

基本信息

批准号：
15405036
负责人：
SUGIMOTO Chihiro
金额：
$ 7.49万
依托单位：
HOKKAIDO UNIVERSITY (2005)Obihiro University of Agriculture and Veterinary Medicine (2003-2004)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15405036/
关键词：
Theileria Babesia Trypanosoma China protozaoa small ruminant 住血微生物

项目摘要

Hemoprotozaon parasite closely related to Chinese Theileria species in Sheep was detected from ticks, Haemaphysalis japonicum infesting on Japanese serow and blood samples of serow by using PCR targeting small subunit ribosomal RNA gene of Chinese Theileria sp. from sheep. However, its infectivity to splenectomized sheep was not confirmed when blood from infected serow was injected. Therefore, transmission of Theileria parasites between domestic and wild ruminants was considered no to occur under natural condition.Several methods based on loop-mediated DNA amplification (LAMP) have been developed for diagnoses of diseases caused by hemoprotozoan parasites, including equine Babesia/Trypanosoma, ovine Babesia/Theileria. Specificity and sensitivity of these tests had been demonstrated to be high as compared with the results obtained by PCR tests. Infection rate of Trypanosoma evansi among horses in Xinjian area exceeded 90 % as determined by LAMP. These LAMP methods will be useful for molecular epidemiological studies of hemoprotozoan parasites in China.

通过使用针对中国theileria sp的小型亚基核糖体RNA基因的PCR，从tick虫中检测到与绵羊中的中国色素物种紧密相关的血op子寄生虫。来自绵羊。然而，当注入感染性血清的血液时，尚未确认其对脾脏绵羊的感染力。因此，在自然条件下认为寄生虫之间的寄生虫在自然状态下不发生。与通过PCR检验获得的结果相比，这些测试的特异性和灵敏度已被证明很高。如灯所确定的，新疆地区马的锥虫感染率超过90％。这些灯的方法将有助于中国血型寄生虫的分子流行病学研究。

项目成果

期刊论文数量（40）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Molecular characterization of Theileria orientalis piroplasm protein encoded by an open reading frame (To ORF2) in a genomic fragment.

基因组片段中开放阅读框 (To ORF2) 编码的东方泰勒虫梨质蛋白的分子特征。

DOI：
发表时间：
2004
期刊：
J Vet Med Sci. 66・8
影响因子：
0
作者：
Kim JY;Yokoyarna N;Kumar S;Inoue N;Fujisaki K;Sugimoto C
通讯作者：
Sugimoto C

Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1

DOI：
10.1016/j.vetpar.2003.10.010
发表时间：
2003-12-30
期刊：
VETERINARY PARASITOLOGY
影响因子：
2.6
作者：
Chahan, B;Gaturaga, I;Xuan, XN
通讯作者：
Xuan, XN

High-level expression of truncated surface antigen P50 of Babesia gibsoni in insect cells by baculovirus and evaluation of its immunogenicity and antigenicity

DOI：
10.1128/cdli.10.4.596-601.2003
发表时间：
2003-07-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
0
作者：
Fukumoto, S;Xuan, XN;Suzuki, H
通讯作者：
Suzuki, H

Detection of antibodies to Neospora caninum in cattle by enzyme-linked immunosorbent assay with truncated NcSRS2 expressed in Escherichia coll.

使用大肠杆菌中表达的截短 NcSRS2，通过酶联免疫吸附测定检测牛体内的犬新孢子虫抗体。

DOI：
发表时间：
2005
期刊：
J Parasitol. 91-1
影响因子：
0
作者：
Gaturaga I;Chahan B;Xuan X;Huang X.Liao M;Fukumoto S;Hirata H;Nishikawa Y;Takashima Y;Suzuki H;Fujisaki K;Sugimoto C.
通讯作者：
Sugimoto C.

Molecular characterization of Theileria orientalis piroplasma protein encoded by an open reading frame(To ORF2) in a genomic fragment.

基因组片段中开放阅读框 (To ORF2) 编码的东方泰勒虫梨原体蛋白的分子特征。