PLANT EXPRESSIONS OF CYTOKINES AND IIST APPILICATIONS FOR ANIMAL DISEASE CONTROL

细胞因子的植物表达和 IIST 在动物疾病控制中的应用

基本信息

批准号：
10556067
负责人：
SUGIMOTO Chihiro
金额：
$ 8.19万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10556067/
关键词：
INTERFERON TRANSGENIC PLANTS CYTOKINE PLANT VIRUS VECTOR

项目摘要

The aim of this study is to express bioactive animal proteins in plants and to apply the recombinant proteins for animal disease control. We used transgenic plant and plant viral vector stems for expression of animal genes.By Agrobacterium-mediated transformation, we introduced cDNAs of two subtypes of human interferon α (IFNα2b and IFNα8). Expression of the introduced genes was confirmed by northern blot analysis and enzyme immunoassay. Ten to 500 IU/g of IFN8 were detected in transgenic potatoes.In bovine leukemia virus infection, tumor necrosis factor α was revealed to be involved in pathogenesis, especially control of viral burden in infected animals. We also clones genes for a tick salivary grand protein, tick serine proteases, and a piroplasm surface antigen that are expected to be expressed in plant and used for method of disease controls.A plant expression system based on clover yellow vein virus has been developed by combining its cDNA and 35S promoter as pC1YVV. Expression of green fluorescence protein by this virus vector was confirmed. INF8 cDNA was introduced into this vector and its expression was tested. However, plant infected with this recombinant virus did not express any product as tested by enzyme immunoassay, provably because the viral vector had not retained the cDNA. Further investigation will be required for stable expression of animal genes in this system.

这项研究的目的是表达植物中的生物活性动物蛋白，并将重组蛋白应用于动物疾病控制。我们使用了转基因植物和植物病毒载体植物来表达动物基因。通过农杆菌介导的转化，我们引入了两个人干扰素α（IFNα2B和IFNα8）的两个亚型的CDNA。通过Northern印迹分析和酶免疫测定确认了引入基因的表达。 Ten to 500 IU/g of IFN8 were detected in transgenic potatoes.In bovine leukemia virus infection, tumor necrosis factor α was revealed to be involved in pathogenesis, especially control of viral burn in infected animals.We also clones genes for a tick salivary grand protein, tick serine proteins, and a piroplasm surface antigen that are expected to be expressed in plant and used for method of disease controls.A基于三叶草黄静脉病毒的植物表达系统是通过将其cDNA和35S启动子与PC1YVV相结合而开发的。证实了该病毒载体对绿色荧光蛋白的表达。将INF8 cDNA引入该载体，并测试其表达。然而，感染这种重组病毒的植物未表达酶免疫测定测试的任何产物，这可能是因为病毒载体尚未保留cDNA。在该系统中，动物基因稳定表达需要进一步研究。