Effects of oral treatment with plant-expressed cytokines for mucosal barrier formation and maturation

植物表达细胞因子口服治疗对粘膜屏障形成和成熟的影响

• 批准号: 15380208
• 负责人: SUGIMOTO Chihiro
• 金额: $ 10.05万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15380208/
• 关键词: Transgenic plants; Plant biothecnology; Mucosal immunity; Oral immunity; 形質転換植物; サイトカイン

The aim of this project is to determine effects of oral treatment with plant-expressed cytokines for mucosal barrier formation and maturation. We also examined protective activity of oral cytokine treatment against pathogens. In a mouse infection model with Listeria monocytogenes, oral treatment with human interferon alfa reduced bacterial burden in spleen and liver. Plant-expressed recombinant protein exhibited similar effect. We obtained information of genes activated with oral treatment with human interferon alfa, and designed primers for the detection of corresponding genes in cattle. Activation of these genes will be used for evaluation of oral interferon treatment in domestic animals. Effect of treatment with transforming growth factor beta was evalualted in mouse infection model with protozoan parasites including Toxoplasma gondii, Babesia microti, Babesia rhodhaini, Plasmodium berghei, Trypanosoma congolense. The treatment reduced parasitemia of T.congolense, which indicates treatment with transforming growth factor beta may activate host defense activity against pathogens.

该项目的目的是确定口服植物表达的细胞因子对粘膜屏障形成和成熟的影响。我们还检查了口服细胞因子对病原体的保护活性。在用单核细胞增生李斯特菌的小鼠感染模型中，用人干扰素ALFA的口服治疗减轻了脾脏和肝脏的细菌负担。植物表达的重组蛋白表现出相似的作用。我们获得了用人干扰素阿尔法（Alfa）口服治疗激活的基因的信息，并设计了用于检测牛相应基因的引物。这些基因的激活将用于评估家畜中的口服干扰素治疗。用转化生长因子β的治疗在小鼠感染模型中用原生动物寄生虫进行评估，包括弓形虫弓形虫，巴贝西亚微动物，巴贝西亚罗德哈尼，伯格氏疟原虫，锥虫菌群，联合锥虫。治疗降低了T. gonolense的寄生虫血症，这表明使用转化生长因子β的治疗可能会激活针对病原体的宿主防御活性。

项目成果

Mammalian cytokines produced in plants

植物中产生的哺乳动物细胞因子

• 发表时间: 2004
• 期刊: Encyclopedia of Plant & Crop Science (Oona Schmid, Editor)(Marcel Dekker, Inc.)
• 作者: Sugimoto;C.
• 通讯作者: C.

Encyclopedia of Plant & Crop Science (Oona Schmid, Editor)

植物百科全书

• 发表时间: 2004
• 作者: Wang;D.W.;Ueda;S.;Uyeda;I.;Yagihashi;H.;Sekiguchi;H.;Tacahashi;Y.;Sato;M.;Ohya;K.;Sugimoto;C.;Matsumura;T.;Chihiro Sugimoto
• 通讯作者: Chihiro Sugimoto

Increase of antibody fiter against Leucocytozoon caulleryi by oral administration of recombinant R7 antigen

口服重组R7抗原提高抗白细胞虫的抗体适​​配度

• 发表时间: 2005
• 期刊: J Vet Med Sci. 67-2
• 作者: T.Kawasaki;F.Nagatsugi;Md.M.Ali;M.Maeda;K.Sugiyama;K.Hori;S.Sasaki;Ito A.
• 通讯作者: Ito A.

Positional effect of gene insertion on genetic stability of a clover yellow vein virus-based expression vector

基因插入位置对三叶草黄脉病毒表达载体遗传稳定性的影响

• 发表时间: 2003
• 期刊: J.Gen.Plant Pathol. 69
• 作者: Wang;D.W.
• 通讯作者: D.W.

Increase of antibody titer against Leucocytozoon caulleryi by oral administration of recombinant R7 antigen

• DOI: 10.1292/jvms.67.211
• 发表时间: 2005-02-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Ito, A;Gotanda, T;Matsumura, T
• 通讯作者: Matsumura, T