中兽医学大肠湿热证的内涵及方证效应相关性研究

Large intestine damp-heat syndrome (LIDHS) in livestock and poultry is a kind of common and frequently-occurring disease. Traditional Chinese veterinary Medicine(TCVM) has good therapeutic effect on this syndrome, but the modern science and material basis of mechanism and treatment effect of this syndrome has not been clear.In this study, LIDHS in animal models plan to establish; Baitouwen decoction and Yujin powder,the two classic prescriptions, chose to treat LIDHS in clinic in TCVM;the relative physiological,biochemical and pathological indicators will detecte;study the dynamic change rules of metabolites in animal model of LIDHS by metabolomics technique; a potential mechanism for the occurrence of LIDHS will study synthetically;efficacy material basis and mechanism of action of treating LIDHS by Baitouwen decoction and Yujin powder will illuminate.On this basis, the physiological,biochemical,pathological indicators,the selected metabolites in animal model of LIDHS and clinical indicators of TCVM will make integration analyses by data mining.Then scientific connotation and principle of treatment of LIDHS in TCV will reveale to make foundation for the diagnosis and treatment of damp-heat syndrome of large intestine, and to provide new ideas and methods for the fundamental research of syndrome in TCVM.

畜禽大肠湿热证是兽医临床常见病、多发病，中兽医对其有较好的治疗作用，但对其证候发生机理和治疗作用的现代科学物质基础不甚清楚。本研究拟建立大肠湿热证动物模型，选用兽医临床治疗大肠湿热证的经典复方白头翁汤和郁金散进行干预，通过相关生理、生化和病理学指标检测，并采用代谢组学方法研究大肠湿热证动物模型体内代谢物的动态变化规律，综合研究大肠湿热证的发生机理，阐明白头翁汤和郁金散治疗大肠湿热证的药效物质基础和作用机理。在此基础上，运用数据挖掘方法，将大肠湿热证动物模型生理、生化、病理学指标和筛选的代谢标志物与传统中兽医对大肠湿热证的诊疗指标进行融合性分析，揭示中兽医学大肠湿热证的科学内涵和治疗原则，为大肠湿热证的诊断和治疗提供理论基础，并为中兽医证候的基础研究提供新的思路和方法。