I-Corps: Development of Integrated Optofluidic ELISA Biosensor Plates

I-Corps：集成光流控 ELISA 生物传感器板的开发

• 批准号: 1340278
• 负责人: Xudong Fan
• 金额: $ 5万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-01 至 2013-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1340278&HistoricalAwards=false
• 关键词: Corps; Development; Integrated; Optofluidic; ELISA

This research team has recently demonstrated integrated optofluidic enzyme-linked immunosorbent assay (ELISA) technology that can tremendously improve the ELISA performance. The proposed project will be focused on the effort to further develop the optofluidic ELISA technology. The integrated optofluidic device is a plastic module that consists of 96 flow-through multi-hole capillaries, which improves the analyte capture efficiency/rate and the signal-to-noise ratio. Currently, it is capable of detecting 1 pg/mL of analytes in only 55 minutes by using only 5 micro-liter sample/reagents, which is already 4-fold, 8-fold, and 20-fold improvement in detection limit, detection time, and sample/reagent consumption, respectively, over the traditional ELISA. Additional 10-fold improvement in sensitivity and analysis speed is expected as the development efforts continue. Significantly, the optofluidic ELISA device is fully compatible with the existing ELISA readers without the need of modification. The proposed device has the potential to be faster, more sensitive, and more cost-effective. Therefore, it could replace the current devices in use. The proposed ELISA device has a societal impact when used in biomedicine. Due to its rapid analysis, it can be used in emergency rooms to provide time-critical information to save lives. The high sensitivity enables early-diagnosis, disease prognosis, and therapeutically monitoring of patients. Finally, significant reduction in reagent volumes translates directly to over 75% savings in healthcare costs. The device is also beneficial to industries and research labs. It expedites the testing turn-around time, cuts the research and development costs, and helps identify molecules at extremely concentrations.

该研究团队最近证明了综合的Optofluidic酶联免疫吸附测定法（ELISA）技术，可以极大地改善ELISA性能。拟议的项目将集中于进一步开发Optofluidic ELISA技术的努力。集成的光荧光设备是一个塑料模块，由96个流通的多孔毛细管组成，可提高分析物捕获效率/速率和信噪比的比率。目前，仅使用5个微型样品/试剂，它仅在55分钟内检测1 pg/mL的分析物，这在检测极限，检测时间和样品/试剂消耗方面分别在传统ELISA上分别为4倍，8倍和20倍。随着开发工作的继续，预计灵敏度和分析速度的10倍提高。值得注意的是，Optofluidic ELISA设备与现有的ELISA读取器完全兼容，而无需修改。提出的设备有可能更快，更敏感和更具成本效益。因此，它可以替换当前使用的设备。拟议的ELISA装置在生物医学中使用时会产生社会影响。由于其快速分析，可以在急诊室中使用它来提供关键的信息以挽救生命。高灵敏度可以使早期诊断，疾病预后以及对患者的治疗监测。最后，试剂量的大幅减少直接减少了医疗费用的75％以上。该设备也对行业和研究实验室有益。它加快了测试转弯时间，削减了研发成本，并有助于以极高的浓度识别分子。