Development of scanning optofluidic cell lasers for highly sensitive cellular and tissue analysis

开发用于高灵敏度细胞和组织分析的扫描光流控细胞激光器

• 批准号: 1607250
• 负责人: Xudong Fan
• 金额: $ 45.43万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-01 至 2020-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1607250&HistoricalAwards=false
• 关键词: Development; scanning; optofluidic; cell; lasers

Title: Development of scanning optofluidic cell lasers for highly sensitive cellular and tissue analysisThe goal of the project: Develop a novel laser based scanning microscope to study cells and tissues.Non-Technical Abstract:The traditional microscope relies on fluorescence to study cells and tissues. However, any small change in cellular activities and tissue structures may not be picked up by the microscope. The proposed project is aimed to develop a laser-based microscope in which laser emission instead of fluorescence is used for detection. Laser emission is much more sensitive than fluorescence. Therefore, small changes in cellular/tissue activities will cause large signals in laser emission, which allows us to study cell and tissues at a more detailed level.The proposed research will provide a new, powerful, and sensitive platform technology complementary to conventional fluorescence based methods for cell/tissue analysis. The detection principle developed through the project can broadly be applicable to any types of laser cavities and bio-species. The proposed research will further provide in-depth understanding of how light interacts with living organisms and biological materials, which will be significant for the development of novel photonic devices. The students in the project will receive interdisciplinary training in optofluidics, photonics, nano/microfabrication, biochemistry, cell biology, and biosensing. The outreach program will educate the general public and train students/teachers from local high schools and non-research-intensive colleges.Technical Abstract:The proposed project is to systematically investigate the biofunctional scanning optofluidic cell laser technology platform and apply it in highly sensitive cell/tissue analysis. The optofluidic laser is an emerging sensing technology that integrates microfluidics, optical microcavity, and gain medium in liquid environment. It employs laser emission as the sensing signal. A small modulation in the gain medium induced by a small change in the underlying biological activities can result in a significant change in the laser output due to optical feedback provided by the laser cavity. Thus, the optofluidic laser based detection is orders of magnitude more sensitive than the fluorescence counterpart. The proposed scanning optofluidic laser is based on a high-performance laser cavity and cells/tissues containing gain media. The laser output will be recorded for detailed understanding of cellular/tissue activities.The proposed research will provide a new, powerful, and sensitive platform technology complementary to conventional fluorescence based methods for cell/tissue analysis. The detection principle developed through the project can broadly be applicable to any types of laser cavities and bio-species. Furthermore, study of the optofluidic cell lasers will provide in-depth understanding of how light interacts with living organisms and biological materials, which will be significant for the development of novel photonic devices. The students in the project will receive interdisciplinary training in optofluidics, photonics, nano/microfabrication, biochemistry, cell biology, and biosensing. The outreach program will educate the general public and train students/teachers from local high schools and non-research-intensive colleges.

标题：用于高度敏感的细胞和组织分析的扫描光流细胞激光器的开发：该项目的目标：开发基于激光的新型扫描显微镜研究细胞和组织。没有技术摘要：传统显微镜依赖荧光来研究细胞和组织。但是，显微镜可能不会拾取细胞活性和组织结构的任何微小变化。拟议的项目旨在开发基于激光的显微镜，其中激光发射而不是荧光用于检测。激光发射比荧光更敏感。因此，细胞/组织活性的微小变化将在激光发射中引起大信号，这使我们能够以更详细的水平研究细胞和组织。拟议的研究将为基于常规荧光的细胞/组织分析方法提供一种新的，功能强大且敏感的平台技术。通过该项目开发的检测原理可以广泛适用于任何类型的激光腔和生物种类。拟议的研究将进一步深入了解光如何与生物体和生物材料相互作用，这对于新型光子设备的开发至关重要。该项目中的学生将接受跨学科的跨学科培训，用于光荧光学，光子学，纳米/微加工，生物化学，细胞生物学和生物传感。外展计划将教育公众和培训来自当地高中和非研究密集型大学的学生/老师。技术摘要：拟议的项目是系统地研究生物功能扫描Optofluidic细胞激光技术平台，并将其应用于高度敏感的细胞/组织分析中。 Optofluidic激光器是一种新兴的传感技术，可在液体环境中整合微流体，光学微腔和增益培养基。它使用激光发射作为传感信号。由于激光腔提供的光学反馈而导致的基础生物活性变化所引起的增益培养基的少量调节可能会导致激光输出的显着变化。因此，基于光流激光的检测是比荧光对应物更敏感的数量级。所提出的扫描光氟化激光基于高性能激光腔和含有增益培养基的细胞/组织。将记录激光输出，以详细了解细胞/组织活动。拟议的研究将为基于传统荧光的基于荧光/组织分析的新型，功能强大且敏感的平台技术提供补充。通过该项目开发的检测原理可以广泛适用于任何类型的激光腔和生物种类。此外，对光氟细胞激光器的研究将对光与生物体和生物材料相互作用的相互作用提供深入的了解，这对于新型光子设备的开发至关重要。该项目中的学生将接受跨学科的跨学科培训，用于光荧光学，光子学，纳米/微加工，生物化学，细胞生物学和生物传感。外展计划将教育公众，并培训来自当地高中和非研究密集型大学的学生/老师。