Point-scanning confocal with area detector

点扫描共焦与区域检测器

• 批准号: 534092360
• 金额: --
• 依托单位: Rheinische Friedrich-Wilhelms-Universität Bonn
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2024
• 资助国家: 德国
• 起止时间: 2023-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/534092360?language=en
• 关键词: Point; scanning; confocal; area; detector

The instrument is going to be used as a research microscope to study cellular processes of live/fixed cells/tissue. The super-resolution detector will allow for exceeding the theoretical resolution limit, down to around 90 nm, and it will provide excellent signal-to-noise ratio and high sensitivity. With this technology we can resolve even smallest cell processes at short times and with low light intensities, which is particular important for live cell imaging. For our correlative light and electron microscopy especially the fast scan times at high resolution are important to allow scans of large volume in a reasonable time but also at a resolution that supports correlation with the EM images. For full support of live cell imaging an environmental chamber is available. The instrument will be placed in a microscopy core facility to serve as a central place for instrument-based services.

该仪器将用作研究显微镜，用于研究活细胞/固定细胞/组织的细胞过程。超分辨率探测器将允许超过理论分辨率极限，低至约 90 nm，并将提供出色的信噪比和高灵敏度。通过这项技术，我们可以在短时间内以低光强度解析最小的细胞过程，这对于活细胞成像尤其重要。对于我们的相关光学和电子显微镜，尤其是高分辨率下的快速扫描时间非常重要，这不仅可以在合理的时间内进行大体积扫描，而且还可以以支持与 EM 图像相关的分辨率。为了全面支持活细胞成像，可以使用环境室。该仪器将放置在显微镜核心设施中，作为基于仪器的服务的中心场所。