IDBR TYPE A: Optofluidic laser array based ultrasensitive ELISA instrument with a large dynamic range

IDBR TYPE A：基于光流控激光阵列的超灵敏 ELISA 仪器，具有大动态范围

• 批准号: 1451127
• 负责人: Xudong Fan
• 金额: $ 38.07万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-02-15 至 2019-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1451127&HistoricalAwards=false
• 关键词: IDBR; TYPE; Optofluidic; laser; array

This NSF IDBR award is made to Prof. Xudong Fan at the University of Michigan, Ann Arbor to develop a new bioassay platform. Significantly improved ELISA performance will certainly benefit nearly all biological/biomedical disciplines as well as industries by detecting or characterizing analytes that would otherwise be undetectable at much lower costs. In addition, the students in the project will receive rigorous technical and entrepreneurial training through lab research and partnership with the Master of Entrepreneurship Program at the University of Michigan. Furthermore, internship at bioassay companies will provide an excellent opportunity for them to receive first-hand experience in an industrial setting. Dissemination of knowledge and instrument will include journal publications, conference presentations, patent filing, public seminars, and demonstration of the prototype at technology tradeshows. In particular, the prototype will be placed in two labs at the Univ. Michigan Medical School and a bioassay company at Ann Arbor for independent verification. Eventually, the dissemination of the instrument will be through commercialization via a start-up company or in partnership with industrial companies.The goal of this project is to develop an optofluidic laser array based enzyme-linked immunosorbent assay (ELISA) instrument that can significantly improve the performance of the current fluorescence based ELISA in detection limit, dynamic range, and sample consumption. Traditional ELISA has been widely used in biological sciences and biomedicine for over half a century. It uses fluorescence as the sensing signal and suffers from low sensitivity, low dynamic range, and relatively large sample consumption. The new ELISA platform in this NSF project performs ELISA inside an optofluidic laser cavity. Instead of fluorescence, the laser emission is used as the sensing signal. In particular, the laser onset time when the laser emission starts to occur is used to quantify the analytes inside the laser cavity. It is expected that 100 to 1000-fold improvement in both detection limit and dynamic range will be achieved in comparison with the traditional ELISA. Meanwhile, the sample consumption will be reduced over 1000-fold.

这项NSF IDBR奖是向密歇根大学安阿伯大学Xudong教授颁发的，以开发一个新的生物测定平台。 通过检测或表征分析物，这些分析物在成本较低时无法检测到的分析物，可以显着改善ELISA的性能几乎可以使几乎所有生物/生物医学学科以及行业受益。此外，该项目的学生将通过实验室研究和与密歇根大学的创业硕士计划进行实验室研究以及合作伙伴关系进行严格的技术和企业家培训。此外，生物测定公司的实习将为他们提供一个在工业环境中获得第一手经验的绝佳机会。知识和工具的传播将包括期刊出版物，会议演讲，专利申请，公共研讨会以及技术贸易展览会原型的演示。特别是，原型将放置在大学的两个实验室中。密歇根州医学院和Ann Arbor的一家生物测定公司进行独立验证。最终，该乐器的传播将通过一家初创公司或与工业公司合作进行商业化。该项目的目的是开发基于基于酶联的酶联免疫吸附测定法（ELISA）的Optofluidic激光阵列（ELISA），该仪器可以显着提高基于当前荧光的ELISA在检测限制范围内的ELISA的性能。半个多世纪以来，传统的ELISA已在生物科学和生物医学中广泛使用。它使用荧光作为感应信号，并且患有低灵敏度，低动态范围和相对较大的样品消耗。该NSF项目中的新ELISA平台在Optofluidic激光腔内执行ELISA。激光发射代替荧光，用作传感信号。特别是，使用激光发射开始的激光发作时间用于量化激光腔内的分析物。与传统ELISA相比，预计将在检测极限和动态范围内提高100至1000倍。同时，样本消耗将减少超过1000倍。