Sensitive Dual Mode Microfluidic Optomechanical Analysis of Biomolecules

生物分子的灵敏双模式微流体光机械分析

• 批准号: 1265164
• 负责人: Xudong Fan
• 金额: $ 37万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1265164&HistoricalAwards=false
• 关键词: Sensitive; Dual; Mode; Microfluidic; Optomechanical

The objective of this award is to harness the light radiation pressure to excite extremely high frequency mechanical vibrations in a microfluidic optomechanical resonator and then apply the microfluidic optomechanical resonator in analyzing biomolecular interactions where both optical and mechanical detection methods will be employed. Different from the state-of-the-art in vibrating microfluidic devices, the microfluidic optomechanical resonator is electrode-less. Both excitation and interrogation of the vibrations are accomplished optically, which breaks the electrical-impedance limit typically seen in current acoustical actuation techniques and enables the microfluidic optomechanical resonator to operate at the acoustical X band (10 GHz). In the project, the microfluidic optomechanical resonator will be analyzed mechanically and optically. Then the microfluidic optomechanical resonator will be fabricated and characterized. Finally, the microfluidic optomechanical resonator will be used to study protein-small molecule interaction and protein-protein interaction where both the mass and the conformational change of biomolecules can be detected.If successful, the results will lead to a fundamental understanding of optomechanics in liquid and a new type of biochemical sensor based on cavity optomechanics. They pave the way to simultaneous acoustical and optical detection of analytes in liquid, which allows for more detailed analysis of biomolecular interactions. Meanwhile, they open a door to many other applications in non-solid phases of matter such as high-resolution ultrasound imaging and optomechanical excitation of superfluids.

该奖项的目的是利用光辐射压力，以在微流体光学机械谐振器中激发极高的频率机械振动，然后在分析光学分子相互作用中应用微流体光学谐振器，其中使用光学和机械检测方法。与振动微流体设备中的最新技术不同，微流体光学谐振器无电极。振动的激发和审问都是光学完成的，这打破了当前声学驱动技术中通常看到的电阻力极限，并使微流体光学机械谐振器能够在声学X频段（10 GHz）处运行。在项目中，将在机械和光学上分析微流体光学谐振器。然后将制造和表征微流体光学谐振器。最后，微流体光学谐振剂将用于研究蛋白质 - 小分子的相互作用和蛋白质 - 蛋白质相互作用，在该蛋白质分子相互作用和蛋白质 - 蛋白质相互作用中，质量和生物分子的构象变化均可被检测到成功。如果成功的结果将导致对液体液体和新型BioChemchemical Sensor Optomanigic opomogrican的基本理解。他们为液体中分析物的同时声学和光学检测铺平了道路，从而可以对生物分子相互作用进行更详细的分析。同时，他们在物质的非稳态阶段（例如高分辨率的超声成像和超级流体的光学激发）打开了许多其他应用的门。