GENOME WIDE SIRNA SCREENING IDENTIFIES NOVEL REGULATORS OF MELANIN SECRETION

全基因组 SIRNA 筛选鉴定出黑色素分泌的新型调节因子

• 批准号: 8362629
• 负责人: TATIANA B KRASIEVA
• 金额: $ 0.51万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362629
• 关键词: Biotechnology; Cells; Confocal Microscopy; Funding; Genes; Goals; Grant; Image; Lasers; Melanins; Melanosomes; Membrane; Microscopy; Movement; National Center for Research Resources; Principal Investigator; Research; Research Infrastructure; Resources; Screening procedure; Small Interfering RNA; Source; Time; United States National Institutes of Health; cost; genome-wide; melanocyte; novel; trafficking

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A genome wide siRNA screen has identified several novel regulators of melanin secretion. The goal of this project is to determine the impact of selected genes on the trafficking of melanosomes within the cells. The goals of the project are to 1) image using standard microscopy the impact of select genes on melanosome trafficking; 2) image using confocal microscopy the impact of select genes on melanosome trafficking (localizaiton adjacent to the membrane); 3) image using confocal microscopy the real time movement of melanosomes within melanocytes.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 基因组宽的siRNA筛选已经确定了黑色素分泌的几个新型调节剂。 该项目的目的是确定所选基因对细胞内黑素体的运输的影响。 该项目的目标是1）使用标准显微镜图像选择基因对黑色素体贩运的影响； 2）使用共聚焦显微镜图像选择基因对黑色素体运输的影响（与膜相邻）； 3）使用共聚焦显微镜图像黑素细胞内黑素体的实时运动。