ROLE OF SMC COMPLEXES IN DNA REPAIR

SMC 复合物在 DNA 修复中的作用

基本信息

批准号：
8362704
负责人：
TATIANA B KRASIEVA
金额：
$ 0.51万
依托单位：
UNIVERSITY OF CALIFORNIA-IRVINE
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-04-01 至 2012-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8362704
关键词：
Biotechnology Chromatin Collaborations Complex DNA Damage DNA Repair Funding Future Gene Expression Regulation Grant Kinetics Laboratory Study Lasers Mitosis Multiprotein Complexes National Center for Research Resources Principal Investigator Regulation Research Research Infrastructure Resources Role Site Source System United States National Institutes of Health cancer therapy cost insight therapeutic target

Biotechnology Chromatin Collaborations Complex DNA Damage DNA Repair Funding Future Gene Expression Regulation Grant Kinetics Laboratory Study Lasers Mitosis Multiprotein Complexes National Center for Research Resources Principal Investigator Regulation Research Research Infrastructure Resources Role Site Source System United States National Institutes of Health cancer therapy cost insight therapeutic target

展开

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. SMC complexes are conserved and essential multiprotein complexes important for higher order chormatin structural organization in mitosis, gene regulation and DNA repair. In collaboration with Dr. Berns' laboratory, we are studying the mechanism and regulation of DNA damage recognition by SMC complexes. We found that near IR laser at the BLI is uniquely suited for detecting the recruitment of one of the SMC complexes to damage sites. We plan to study the recruitment kinetics and factor requirement using the system. The study will provides important insight into the role of chromatin regulators in DNA repair, which may serve as possible therapeutic targets for cancer treatment in the future.

该副本是利用资源的众多研究子项目之一由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持而且，副投影的主要研究员可能是其他来源提供的包括其他NIH来源。列出的总费用可能代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 SMC复合物是保守的，必需的多蛋白络合物对于有丝分裂，基因调节和DNA修复中的高阶结构组织重要。与伯恩斯博士的实验室合作，我们正在研究SMC复合物对DNA损伤识别的机制和调节。我们发现，BLI的IR激光附近非常适合检测其中一种SMC复合物损坏位点的募集。我们计划使用系统研究招聘动力学和因素要求。该研究将为染色质调节剂在DNA修复中的作用提供重要的见解，DNA修复中可能是对癌症治疗的可能治疗靶标。