TRACHEAL CARTILAGE RESHAPING

气管软骨重塑

• 批准号: 8362636
• 负责人: TATIANA B KRASIEVA
• 金额: $ 1.4万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362636
• 关键词: Anterior; Biotechnology; Cartilage; Devices; Funding; Germany; Grant; Inflammation; Lasers; Measures; Mechanics; Microscope; Morbidity - disease rate; National Center for Research Resources; New Zealand; Operative Surgical Procedures; Oryctolagus cuniculus; Principal Investigator; Property; Research; Research Infrastructure; Resources; Scanning; Source; Stenosis; Techniques; Testing; Tissue Viability; Tissues; United States National Institutes of Health; cost; design; dosimetry; instrument; repaired

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. INTRODUCTION:The usual surgical repair techniques (so-called "open technique") for anterior tracheal wall collapse are often associated with a high rate of morbidity, and prolonged stenting can aggravate the ongoing tracheal inflammation and serve as a potential source for a new stenosis. We are currently studying the laser dosimetry and the design of the surgical device for endoscopic laser cartilage reshaping minimizing the thermal damage of cartilage tissue. OBJECTIVE: In order to provide a better understanding of laser-induced tracheal cartilage reshaping, we examined the relationships between the tissue viability and the laser dosimetry as well as the change in mechanical property due to endoscopic laser exposure. In addition, we design an effective endoscopic surgical device for laser tracheal cartilage reshaping. MATERIALS AND METHODS: Tracheal cartilage extracted from New Zealand rabbits, were exposed to diode laser (wavelength =1450nm, 0.03-0.14W/mm2, Candela Corp. MA) for 3-5 seconds. The change in the mechanical property was measured using an ELF 3200 mechanical testing instrument (EnduraTEC, Minnetonka, MN) and its viability was determined using a laser scanning microscope (LSM510 Meta, Carl Zeiss Inc., Germany).

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 简介：前气管壁塌陷的通常的手术修复技术（所谓的“开放技术”）通常与高发病率有关，并且长时间支架的延长会加剧持续的气管炎症，并作为新狭窄的潜在来源。我们目前正在研究激光剂量法和外科手术装置的设计，用于内窥镜激光软骨的重塑，以最大程度地减少软骨组织的热损伤。目的：为了更好地了解激光诱导的气管重塑，我们检查了组织生存能力与激光剂量测定法之间的关系，以及由于内窥镜激光暴露而引起的机械性能的变化。此外，我们设计了一种有效的内窥镜手术装置，用于重塑激光气管。材料和方法：从新西兰兔子提取的气管，暴露于二极管激光器（波长= 1450nm，0.03-0.14W/mm2，Candela Corp. MA）3-5秒。使用ELF 3200机械测试仪器（Enduratec，Minnetonka，MN）测量了机械性能的变化，并使用激光扫描显微镜（LSM510 Meta，Carl Zeiss Inc.，德国）确定其可行性。