IN VIVO OPTICAL DIAGNOSIS OF ORAL MALIGNANCY IN HUMAN SUBJECTS

人类受试者口腔恶性肿瘤的体内光学诊断

• 批准号: 8362606
• 负责人: TATIANA B KRASIEVA
• 金额: $ 0.51万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362606
• 关键词: Biopsy; Biotechnology; Blinded; Blood Vessels; Cellular Structures; Cessation of life; Collagen; Data; Detection; Diagnosis; Diagnostic; Diagnostic Imaging; Dysplasia; Ensure; Epithelial; Fluorescence; Funding; Grant; Histopathology; Image; Lasers; Malignant - descriptor; Malignant Neoplasms; Maps; Methods; Modality; National Center for Research Resources; Optical Coherence Tomography; Optics; Oral Diagnosis; Pathology; Patients; Principal Investigator; Quality of life; ROC Curve; Research; Research Infrastructure; Research Personnel; Resources; Screening for cancer; Sensitivity and Specificity; Site; Source; Specificity; Structure; Surface; Techniques; Technology; Testing; Tissues; United States National Institutes of Health; cost; human subject; in vivo; malignant mouth neoplasm; multi-photon; oral dysplasia; oral lesion; oral premalignancy; oral tissue; second harmonic; statistics; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Objectives: Early detection of cancer and its curable precursors remains the best way to ensure patient survival and quality of life. Despite significant advances in treatment, oral cancer still results in 10,000 U.S. deaths annually, mainly due to the late detection of most oral lesions. Specific aim: Using a combination of non-invasive optical in vivo technologies to test a multi-modality approach to non-invasive diagnostics of oral premalignancy and malignancy in human subjects. Methods: In 30 patients with healthy, dysplastic, and/or malignant oral tissues, in vivo optical coherence tomography (OCT) and optical Doppler tomography (ODT) mapped epithelial, subepithelial and vascular change in specific, marked sites prior to routine biopsy. Ex vivo multi-wavelength multi-photon (MPM) and second harmonic generated (SHG) fluorescence techniques provided parallel data on surface and subsurface tissue structure, specifically collagen presence and structure, cellular presence, and vasculature. Images were diagnosed by 2 blinded, pre-standardized investigators using a standardized scale from 0-6 for all modalities. Histopathological sections were prepared and pathology evaluated on a scale of 0-6. ANOVA techniques compared imaging diagnostics with histopathology. 95% confidence limits of the sensitivity and specificity were established for the diagnostic capability of OCT/ODT+ MPM/SHG using ROC curves and kappa statistics. Results: Imaging data were reproducibly obtained with good accuracy. Dysplasia- and malignancy-related structural and vascular changes were clearly visible to tissue depths of 2mm. Sensitivity (OCT/ODT alone: 72-89%; OCT+MPM/SHG: 76-90%) and specificity (OCT alone: 68-84%;OCT+MPM/SHG: 74-88%) compared well with conventional techniques. Conclusions: OCT/ODT and MPM/SHG are promising non-invasive in vivo diagnostic modalities for oral dysplasia and malignancy.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 目标：早期发现癌症及其可治愈的前体仍然是确保患者生存和生活质量的最佳方法。尽管治疗方面取得了重大进展，但口腔癌仍会每年导致10,000例美国死亡，这主要是由于大多数口腔病变迟到。具体目的：使用非侵入性光学在体内技术的组合来测试人类受试者口服预现象和恶性肿瘤的多模式方法。方法：在30例健康，发育不良和/或恶性口腔组织的患者中，体内光学相干断层扫描（OCT）和光学多普勒断层扫描（ODT）映射的上皮，上皮下皮下皮和血管变化。离体多波长多光子（MPM）和第二次谐波（SHG）荧光技术在表面和地下组织结构上提供了并行数据，特别是胶原蛋白的存在和结构，细胞的存在和脉管系统。图像通过2个盲目的，预标准化的研究人员使用标准化量表从0-6诊断为所有模式。制备组织病理学切片，并以0-6的比例评估病理学。方差分析技术将成像诊断与组织病理学进行了比较。使用ROC曲线和KAPPA统计数据，建立了95％的敏感性和特异性置信度限制，以实现OCT/ODT+ MPM/SHG的诊断能力。结果：成像数据以良好的精度可重复获得。 2mm的组织深度清楚地看到了与发育不良和恶性相关的结构和血管变化。敏感性（单独使用OCT/ODT：72-89％； OCT+MPM/SHG：76-90％）和特异性（仅十月：68-84％； OCT+MPM/SHG：74-88％）与常规技术相比。结论：OCT/ODT和MPM/SHG是口腔发育不良和恶性肿瘤的体内诊断方式有望的。