Animal Model Core

动物模型核心

基本信息

批准号：
10224227
负责人：
Gerard J Nau
金额：
$ 38.52万
依托单位：
MIRIAM HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2023-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10224227
关键词：
Animal Experiments Animal Model Animals Antibiotics Antimicrobial Resistance Bacteria Biometry Biotechnology Blinded Centers of Research Excellence Collaborations Communities Consultations Data Development Experimental Designs Guidelines Harvest Hospitals Host Defense Human IACUC Immune response Individual Infection Institution Investigation Laboratory Research Lead Longevity Longitudinal Studies Measures Modeling Mus Neutrophil Activation Organism Outcome Outcome Measure Parents Pathogenesis Pharmacologic Substance Pilot Projects Pneumonia Positioning Attribute Preclinical Testing Process Program Research Project Grants Protocols documentation Reproducibility Research Personnel Research Project Grants Resources Schedule Standardization Supervision Techniques Testing Therapeutic Training Translating United States National Institutes of Health Work Writing antibiotic tolerance antimicrobial drug data acquisition design drug discovery drug resistant pathogen experimental study follow-up imaging system in vivo in vivo imaging system insight instrument microbiome mouse model novel novel therapeutics pre-clinical assessment preclinical study recruit sound trafficking transcriptome treatment group

Animal Experiments Animal Model Animals Antibiotics Antimicrobial Resistance Bacteria Biometry Biotechnology Blinded Centers of Research Excellence Collaborations Communities Consultations Data Development Experimental Designs Guidelines Harvest Hospitals Host Defense Human IACUC Immune response Individual Infection Institution Investigation Laboratory Research Lead Longevity Longitudinal Studies Measures Modeling Mus Neutrophil Activation Organism Outcome Outcome Measure Parents Pathogenesis Pharmacologic Substance Pilot Projects Pneumonia Positioning Attribute Preclinical Testing Process Program Research Project Grants Protocols documentation Reproducibility Research Personnel Research Project Grants Resources Schedule Standardization Supervision Techniques Testing Therapeutic Training Translating United States National Institutes of Health Work Writing antibiotic tolerance antimicrobial drug data acquisition design drug discovery drug resistant pathogen experimental study follow-up imaging system in vivo in vivo imaging system insight instrument microbiome mouse model novel novel therapeutics pre-clinical assessment preclinical study recruit sound trafficking transcriptome treatment group

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项目摘要

Project Summary: Animal Models Core Studies of antibiotic tolerance and of new therapies to treat antibiotic-resistant pathogens must, at some point, be evaluated in mammalian models of infection. These pre-clinical assessments are a fundamental step to determining whether treatments might be translated to human studies. Moreover, the complexities of the mammalian immune response can be queried to gain insight into the basic pathobiology of infections and host defenses. The Animal Models Core of the Center for Antimicrobial Resistance and Therapeutic Discovery (CARTD) will enable pre-clinical studies in mice to facilitate rapid, high-quality data acquisition. The Core will provide essential input, along with that from our biostatistics expert, to aid in experimental design that enhances the robustness of the results. The Core Director will train the Junior Investigators and assist them in obtaining the institutional approvals necessary to conduct animal experiments. The Core will maintain a portfolio of techniques directly related to the experimental objectives of the research projects, as well as expand the repertoire to diversify the research projects and to recruit investigators from beyond the CARTD. The Core’s design includes blinding of treatment groups so that observers make unbiased assessments of the animals throughout the experiment. The Core’s staff will conduct experiments in conjunction with the laboratories of the research and pilot projects. Importantly, the Core will be responsible for managing the new in vivo imaging system recently procured by our institution. This imaging system enhances the robustness and reproducibility of the studies by longitudinal follow-up of individual animals while reducing the overall numbers of animals that are required in any given experiment. The Animal Models Core’s functions are defined by the following specific aims: 1. To provide consultation and assistance in planning experimental design, strain selection, and outcomes analysis. 2. To conduct infection and treatment experiments in collaboration with investigators from the Junior Investigator and Pilot Project research programs, as well as outside researchers. 3. To supervise, schedule, maintain, and operate the PerkinElmer IVIS Lumina III in vivo imaging system.

项目摘要：动物模型核心对治疗抗生素耐药病原体的抗生素耐受性和新疗法的研究必须在某个时候在感染的哺乳动物模型中进行评估。这些临床前评估是确定是否可以将治疗转化为人类研究。而且，可以查询哺乳动物免疫反应以深入了解感染和宿主的基本病理学防御。动物模型抗菌耐药性和治疗发现中心的核心（CARTD）将使小鼠进行临床前研究，以促进快速，高质量的数据获取。核心意愿提供基本的输入以及我们的生物统计学专家，以帮助实验设计增强结果的鲁棒性。核心主任将培训初级调查员并协助他们获得进行动物实验所需的机构批准。核心将保持技术组合与研究项目的实验目标直接相关，以及扩展曲目，以使研究项目多样化，并从CARTD以外招募研究人员。核心的设计包括对治疗组的盲目，以便观察者对整个实验中的动物。核心人员将与研究和试点项目的实验室。重要的是，核心将负责管理新的我们机构最近采购的体内成像系统。这个成像系统增强了鲁棒性和研究通过纵向随访的单个动物的可重复性，同时减少总数在任何给定实验中都需要的动物。动物模型核心的功能由以下具体目的：1。为计划实验设计提供咨询和帮助选择和结果分析。 2。与初级研究员和试点项目研究计划以及外部研究人员的研究人员。 3。监督，安排，维护和操作Perkinelmer Ivis Lumina III体内成像系统。